Effects of Joint Immobilization on Changes in Myofibroblasts and Collagen in the Rat Knee Contracture Model
Effects of Joint Immobilization on Changes in Myofibroblasts and Collagen in the Rat Knee Contracture Model
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DOI：
10.1002/jor.23498
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发表时间：
2017-09-01
影响因子：
2.8
通讯作者：
Okita, Minoru
中科院分区：
文献类型：
--
作者：
Sasabe, Ryo;Sakamoto, Junya;Okita, Minoru
本研究的目的是研究的时间依赖性变化的关节囊纤维化的发展和制动后关节囊中的肌成纤维细胞的数量，使用大鼠膝关节挛缩模型。制动组：双侧膝关节屈曲固定1、2、4周。每个制动期饲养未处理大鼠（对照组）。进行组织学分析以评估关节囊中胶原蛋白的量和密度的变化。原位杂交检测I型和III型胶原mRNA的变化。免疫组化法检测关节囊肌成纤维细胞的数量。与对照组相比，制动组1周内胶原含量增加，2周内胶原密度增加。制动组I型胶原mRNA阳性细胞数在所有时间点均增加。然而，III型胶原mRNA阳性细胞数并没有增加。制动组肌成纤维细胞在各时间点均较对照组显著增加，且随制动时间延长而显著增加。这些结果表明，关节囊纤维化与过度表达的I型胶原蛋白的发生和发展在1周内制动后，和肌成纤维细胞的增加有关的关节囊纤维化的机制。研究结果表明，需要一种针对与肌成纤维细胞增加相关的I型胶原积累的治疗方法。(C)2017骨科研究学会。出版社：Wiley Periodicals，Inc.
The purpose of this study was to examine the time-dependent changes in the development of joint capsule fibrosis and in the number of myofibroblasts in the joint capsule after immobilization, using a rat knee contracture model. Both knee joints were fixed in full flexion for 1, 2, and 4 weeks (immobilization group). Untreated rats were bred for each immobilization period (control group). Histological analysis was performed to evaluate changes in the amount and density of collagen in the joint capsule. The changes in type I and III collagen mRNA were examined by in situ hybridization. The number of myofibroblasts in the joint capsule was assessed by immunohistochemical methods. In the immobilization group, the amount of collagen increased within 1 week and the density of collagen increased within 2 weeks, as compared with that in the control group. Type I collagen mRNA-positive cell numbers in the immobilization group increased at all time points. However, type III collagen mRNA-positive cell numbers did not increase. Myofibroblasts in the immobilization group significantly increased compared with those in the control group at all time points, and they increased significantly with the period of immobilization. These results suggest that joint capsule fibrosis with overexpression of type I collagen occurs and progresses within 1 week after immobilization, and an increase in myofibroblasts is related to the mechanism of joint capsule fibrosis. The findings suggest the need for a treatment targeting accumulation of type I collagen associated with an increase in myofibroblasts. (C) 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.