Actinomycin D induced DNase I hypersensitivity and asymmetric structure transmission in a DNA hexadecamer.
Actinomycin D induced DNase I hypersensitivity and asymmetric structure transmission in a DNA hexadecamer.
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Actinomycin D 诱导 DNA 十六聚体中的 DNase I 超敏性和不对称结构传递。
DOI：
10.1093/nar/19.4.871
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发表时间：
1991
影响因子：
14.9
通讯作者：
Lane,MJ
中科院分区：
文献类型：
--
作者：
Bishop,KD;Borer,PN;Huang,YQ;Lane,MJ
DNase I裂解速率和核磁共振化学位移显示，当药物结合时，插层放线菌素D分子远端的DNA序列会发生变化。这两组观察都表明，这些变化的来源是DNA介导的结构性反应。核磁共振结果表明，反应优先地从药物结合部位的5‘到3’方向传递。两条链对配体引起的构象变化的不同反应立即表明了一种区分DNA正义和反义链的机制。
DNase I cleavage rates and nmr chemical shifts are shown to change for DNA sequences distal to an intercalated actinomycin D molecule in a duplex hexadecamer upon drug binding. Both sets of observations suggest that the source of these changes is a DNA-mediated structural response. The nmr results imply the response is transmitted preferentially in a 5′-to-3′ direction from the drug binding site. An inequivalent response of the two strands to a ligandinduced conformational change immediately suggests a mechanism for distinguishing the sense and antisense strands of DNA.