Bilayer sheet protrusions and budding from bilayer membranes induced by hydrolysis and condensation reactions
Bilayer sheet protrusions and budding from bilayer membranes induced by hydrolysis and condensation reactions
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DOI：
10.1039/c7sm02326j
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发表时间：
2018-02-28
期刊：
影响因子：
3.4
通讯作者：
Noguchi, Hiroshi
中科院分区：
文献类型：
--
作者：
Nakagawa, Koh M.;Noguchi, Hiroshi
利用粗粒度分子动力学模拟研究了两亲分子水解和缩合反应引起的平板双层膜和囊泡的形状变化。水解和缩合反应分别导致两亲分子的形成和解离。囊泡的内外小叶之间的不对称反应可以在小叶之间运输两亲分子。结果发现，所产生的两个小叶之间的面积差异诱导双层片突起（BP）和出芽在低减少体积的囊泡，而BP只发生在高减少体积。这两种类型的转换的概率取决于与膜相比的周围流体的剪切粘度以及反应速率。较高的周围流体粘度导致更多的BP形成。疏水反应产物的不均匀空间分布形成BP形成的核，并且产物的更快扩散促进BP形成。我们的研究结果表明，在实验中，粘度的调整是重要的控制膜的形状转换。
Shape transformations of flat bilayer membranes and vesicles induced by hydrolysis and condensation reactions of amphiphilic molecules are studied using coarse-grained molecular dynamics simulations. The hydrolysis and condensation reactions result in the formation and dissociation of amphiphilic molecules, respectively. Asymmetric reactions between the inner and outer leaflets of a vesicle can transport amphiphilic molecules between the leaflets. It is found that the resulting area difference between the two leaflets induces bilayer sheet protrusion (BP) and budding at low reduced volumes of the vesicles, whereas BP only occurs at high reduced volumes. The probabilities of these two types of transformations depend on the shear viscosity of the surrounding fluids compared to the membrane as well as the reaction rates. A higher surrounding fluid viscosity leads to more BP formation. The inhomogeneous spatial distribution of the hydrophobic reaction products forms the nuclei of BP formation, and faster diffusion of the products enhances BP formation. Our results suggest that adjustment of the viscosity is important to control membrane shape transformations in experiments.