Comparison between solution-phase stability and gas-phase kinetic stability of oligodeoxynucleotide duplexes
Comparison between solution-phase stability and gas-phase kinetic stability of oligodeoxynucleotide duplexes
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DOI：
10.1002/jms.141
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发表时间：
2001-04-01
影响因子：
2.3
通讯作者：
De Pauw, E
中科院分区：
文献类型：
--
作者：
Gabelica, V;De Pauw, E
在加热毛细管电喷雾离子源中，利用源碰撞诱导解离（CID）研究了不同16-mer寡核苷酸双链体的相对动力学稳定性。并与紫外分光光度法测定的热变性法测定的相对稳定性进行了比较。结果清楚地表明，存在于溶液中的氢键和碱基堆积相互作用都保持在气相中。这表明电喷雾过程保留了DNA的双螺旋结构。一步一步打开的双螺旋结构提出的气相解离，竞争的共价键裂解的基地。我们还提请注意这样一个事实，即通过源CID，探测的是络合物的动力学稳定性。特别是，这意味着只能比较相同大小的复合物。版权所有（C）2001约翰威利父子有限公司
The relative kinetic stabilities of different 16-mer oligonucleotide duplexes were investigated by source collision-induced dissociation (CID) in a heated capillary electrospray ion source. They were compared with the relative stabilities in solution obtained by thermal denaturation monitored by UV spectrophotometry. The results clearly show that both hydrogen bonding and base stacking interactions that are present in solution are maintained in the gas phase. This suggests that the electrospray process preserves the double-helix structure of DNA. A step by step opening of the double helix structure is proposed for the gas-phase dissociation, competing with the covalent bond cleavage of bases. We also draw attention to the fact that by source CID, it is the kinetic stability of the complexes that is probed. In particular, this implies that only complexes of the same size can be compared. Copyright (C) 2001 John Wiley & Sons, Ltd.