A Kunitz Protease Inhibitor from Dermacentor variabilis, a Vector for Spotted Fever Group Rickettsiae, Limits Rickettsia montanensis Invasion
A Kunitz Protease Inhibitor from Dermacentor variabilis, a Vector for Spotted Fever Group Rickettsiae, Limits Rickettsia montanensis Invasion
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DOI：
10.1128/iai.00362-10
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发表时间：
2011-01-01
影响因子：
3.1
通讯作者：
Azad, Abdu F.
中科院分区：
文献类型：
--
作者：
Ceraul, Shane M.;Chung, Ashley;Azad, Abdu F.
蜱立克次体共生的一个决定性方面是每一个伙伴为确保自己的生存而采用的分子策略。蜱虫必须控制立克次体的定植以避免立即死亡。在目前的研究中，我们表明，立克次体的丰度在蜱中肠增加，一旦库尼茨型丝氨酸蛋白酶抑制剂的表达，从美国狗蜱（Dermacentor variabilis）（DvKPI）被小干扰RNA（siRNA）抑制。一系列体外侵袭试验表明，DvKPI限制立克次体在宿主细胞进入过程中的定殖。有趣的是，我们观察到DvKPI与立克次体在体外以及在蜱中肠。总的来说，我们的数据表明，DvKPI限制宿主细胞的入侵，可能是通过与细菌的关联。
A defining facet of tick-Rickettsia symbioses is the molecular strategy employed by each partner to ensure its own survival. Ticks must control rickettsial colonization to avoid immediate death. In the current study, we show that rickettsial abundance in the tick midgut increases once the expression of a Kunitz-type serine protease inhibitor from the American dog tick (Dermacentor variabilis) (DvKPI) is suppressed by small interfering RNA (siRNA). A series of in vitro invasion assays suggested that DvKPI limits rickettsial colonization during host cell entry. Interestingly, we observed that DvKPI associates with rickettsiae in vitro as well as in the tick midgut. Collectively, our data demonstrate that DvKPI limits host cell invasion by Rickettsia montanensis, possibly through an association with the bacterium.