Adaptive evolution and demographic history contribute to the divergent population genetic structure of Potato virus Y between China and Japan.
Adaptive evolution and demographic history contribute to the divergent population genetic structure of Potato virus Y between China and Japan.
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DOI：
10.1111/eva.12459
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发表时间：
2017-04
影响因子：
4.1
通讯作者：
Zhan J
中科院分区：
文献类型：
--
作者：
Gao F;Zou W;Xie L;Zhan J
马铃薯Y病毒(PVY)是一种重要的植物病原菌，给马铃薯生产造成了巨大的经济损失。关于病原体的种群遗传结构和进化生物学的知识，特别是在跨国范围内的知识是有限的，但对于制定可持续管理计划至关重要。本研究利用中国和日本马铃薯分离物的127个第一蛋白(P1)和137个外壳蛋白(CP)序列对PVY的群体遗传结构和分子进化进行了研究。两国种群间存在较高的遗传分化，日本种群在两个基因上的核苷酸多样性均高于中国，两个基因串联序列的KST值为0.216。这两个国家的序列根据它们的地理来源聚集在一起。进一步的分析表明，PVY种群的空间遗传结构可能是由病原菌的种群动态和栖息地异质性产生的自然选择造成的。在大多数多态位点上检测到纯化选择，但也检测到一些支系特有密码子处于正选择状态。在过去的几十年里，PVY在中国经历了种群扩张，而在日本，该病原菌的种群规模保持相对稳定。
Potato virus Y (PVY) is an important plant pathogen causing considerable economic loss to potato production. Knowledge of the population genetic structure and evolutionary biology of the pathogen, particularly at a transnational scale, is limited but vital in developing sustainable management schemes. In this study, the population genetic structure and molecular evolution of PVY were studied using 127 first protein (P1) and 137 coat protein (CP) sequences generated from isolates collected from potato in China and Japan. High genetic differentiation was found between the populations from the two countries, with higher nucleotide diversity in Japan than China in both genes and a KST value of .216 in the concatenated sequences of the two genes. Sequences from the two countries clustered together according to their geographic origin. Further analyses showed that spatial genetic structure in the PVY populations was likely caused by demographic dynamics of the pathogen and natural selection generated by habitat heterogeneity. Purifying selection was detected at the majority of polymorphic sites although some clade‐specific codons were under positive selection. In past decades, PVY has undergone a population expansion in China, whereas in Japan, the population size of the pathogen has remained relatively constant.