THE ETS TRANSCRIPTION FACTORS ENCODED BY THE DROSOPHILA GENE POINTED DIRECT GLIAL-CELL DIFFERENTIATION IN THE EMBRYONIC CNS
THE ETS TRANSCRIPTION FACTORS ENCODED BY THE DROSOPHILA GENE POINTED DIRECT GLIAL-CELL DIFFERENTIATION IN THE EMBRYONIC CNS
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DOI：
10.1016/0092-8674(94)90581-9
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发表时间：
1994-07-15
期刊：
影响因子：
64.5
通讯作者：
KLAMBT, C
中科院分区：
文献类型：
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作者：
KLAES, A;MENNE, T;KLAMBT, C
果蝇基因POINT(PNT)编码Ets家族的两个转录因子(P1和P2)，在胚胎中枢神经系统中仅在神经胶质细胞中发现。PNT功能的丧失导致低分化的神经胶质细胞和MP2神经元中神经元抗原22C10的表达显著减少，这些神经元与表达PNT(P1)的纵向神经胶质细胞密切相互作用。PNT(P1)RNA的异位表达迫使更多的CNS细胞进入神经胶质分化途径。有趣的是，额外的神经胶质样细胞通常位于异位表达神经元抗原22C10的细胞的两侧。因此，PNT功能丧失和功能获得表型都表明神经胶质细胞能够诱导邻近神经元上22C10的表达。细胞移植实验进一步证实了这一点。因此，PNT不仅是神经胶质分化所必需的，而且是足够的。
The Drosophila gene pointed (pnt) encodes two putative transcription factors (P1 and P2) of the Ets family, which in the embryonic CNS are found exclusively in glial cells. Loss of pnt function leads to poorly differentiated glial cells and a marked decrease in the expression of the neuronal antigen 22C10 in the MP2 neurons, which are known to interact intimately with the pnt(P1)-expressing longitudinal glial cells. Ectopic expression of pnt(P1) RNA forces additional CNS cells to enter the glial differentiation pathway. Interestingly, the additional glial-like cells are often flanked by cells that ectopically express the neuronal antigen 22C10. Therefore, both the pnt loss-of-function as well as the gain-of-function phenotype suggest that glial cells are able to induce 22C10 expression on neighboring neurons. This was further verified by cell transplantation experiments. Thus, pnt is not only required but also sufficient for several aspects of glial differentiation.