In vitro degradation of porous poly(propylene fumarate)/poly(DL-lactic-co-glycolic acid) composite scaffolds
In vitro degradation of porous poly(propylene fumarate)/poly(DL-lactic-co-glycolic acid) composite scaffolds
复制标题
DOI：
10.1016/j.biomaterials.2004.09.012
复制
发表时间：
2005-06-01
期刊：
影响因子：
14
通讯作者：
Mikos, AG
中科院分区：
文献类型：
--
作者：
Hedberg, EL;Shih, CK;Mikos, AG
本研究调查了含有聚（DL-乳酸-乙醇酸）（PLGA）和聚（乙二醇）（PEG）混合物微粒的多孔聚（富马酸丙烯酯）（基于 PPF 的）复合材料在 37 ℃、pH 7.4 磷酸盐缓冲盐水中为期 26 周的体外降解情况。使用部分因子设计，制造了四种复合支架配方，其微粒的PEG含量、复合材料的微粒质量分数以及支架配方的初始可浸出致孔剂含量不同。不含微粒的 PPF 支架采用不同的可浸出致孔剂含量制成，用作对照。对于长度为 13 毫米、直径为 6.5 毫米的圆柱形样品，研究了 PPF 支架中包含 PLGA/PEG 微粒的效果以及复合材料配方的变化对支架质量、几何形状、吸水率、机械性能和孔隙率的影响。复合支架组合物影响聚合物质量、支架长度和直径的损失程度，26周内聚合物质量的最大损失等于15+/-5%。然而，没有任何制剂随着时间的推移表现出压缩模量或峰值压缩强度的任何变化。此外，通过水银孔隙率测定法和微型计算机断层扫描确定的样品孔隙率在本研究期间没有变化。这些结果表明，微粒载体可以整合到 PPF 支架中，用于生物活性分子的局部递送，而在体外长达 26 周内不会改变支架的机械或结构特性。 (C) 2004 Elsevier Ltd. 保留所有权利。
This study investigated the in vitro degradation of porous poly(propylene fumarate) (PPF-based) composites incorporating microparticles of blends of poly(DL-lactic-eo-glycolic acid) (PLGA) and poly(ethylene glycol) (PEG) during a 26-week period in pH 7.4 phosphate-buffered saline at 37 degreesC. Using a fractional factorial design, four formulations of composite scaffolds were fabricated with varying PEG content of the microparticles, microparticle mass fraction of the composite material, and initial leachable porogen content of the scaffold formulations. PPF scaffolds without microparticles were fabricated with varying leachable porogen content for use as controls. The effects of including PLGA/PEG microparticles in PPF scaffolds and the influence of alterations in the composite formulation on scaffold mass, geometry, water absorption, mechanical properties and porosity were examined for cylindrical specimens with lengths of 13 mm and diameters of 6.5 mm. The composite scaffold composition affected the extent of loss of polymer mass, scaffold length, and diameter, with the greatest loss of polymer mass equal to 15+/-5% over 26 weeks. No formulation, however, exhibited any variation in compressive modulus or peak compressive strength over time. Additionally, sample porosity, as determined by both mercury porosimetry and micro-computed tomography did not change during the period of this study. These results demonstrate that microparticle carriers can be incorporated into PPF scaffolds for localized delivery of bioactive molecules without altering scaffold mechanical or structural properties up to 26 weeks in vitro. (C) 2004 Elsevier Ltd. All rights reserved.