The local immune response of mice after Helicobacter suis infection: strain differences and distinction with Helicobacter pylori
The local immune response of mice after Helicobacter suis infection: strain differences and distinction with Helicobacter pylori
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DOI：
10.1186/1297-9716-43-75
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发表时间：
2012-10-29
影响因子：
4.4
通讯作者：
Haesebrouck, Freddy
中科院分区：
文献类型：
--
作者：
Flahou, Bram;Van Deun, Kim;Haesebrouck, Freddy
幽门螺杆菌（H.）猪的胃定植，是最普遍的胃非H。幽门螺杆菌在人类中的种类。有限的信息是可用的宿主免疫反应后，感染这种代理，它是未知的，如果不同的H之间存在的毒力差异。猪种。因此，BALB/c和C57BL/6小鼠被用于比较在实验感染9种不同的H.猪种。所有菌株都能够在小鼠胃中持续存在，但接种后59天C57BL/6小鼠胃中的定殖细菌数量高于BALB/c小鼠。全是H。suis菌株引起白细胞介素（IL）-17的上调，这在BALB/c小鼠中更明显。这种上调与定殖细菌的数量呈负相关。大多数菌株还引起调节性IL-10的上调，与BALB/c小鼠中的定殖正相关。仅在C57 BL/6小鼠中观察到IL-1 β的上调。IFN-γ mRNA水平的增加从未被检测到，而大多数H。suis菌株主要在BALB/c小鼠中引起Th2特征细胞因子IL-4的上调。总之，小鼠品系的遗传背景对不同H. suis菌株和它们引起的免疫反应。Th17应答占优势，伴有轻度Th2应答，与H.幽门感染
Helicobacter (H.) suis colonizes the stomach of pigs and is the most prevalent gastric non-H. pylori Helicobacter species in humans. Limited information is available on host immune responses after infection with this agent and it is unknown if variation in virulence exists between different H. suis strains. Therefore, BALB/c and C57BL/6 mice were used to compare colonization ability and gene expression of various inflammatory cytokines, as determined by real-time PCR, after experimental infection with 9 different H. suis strains. All strains were able to persist in the stomach of mice, but the number of colonizing bacteria at 59 days post inoculation was higher in stomachs of C57BL/6 mice compared to BALB/c mice. All H. suis strains caused an upregulation of interleukin (IL)-17, which was more pronounced in BALB/c mice. This upregulation was inversely correlated with the number of colonizing bacteria. Most strains also caused an upregulation of regulatory IL-10, positively correlating with colonization in BALB/c mice. Only in C57BL/6 mice, upregulation of IL-1 beta was observed. Increased levels of IFN-gamma mRNA were never detected, whereas most H. suis strains caused an upregulation of the Th2 signature cytokine IL-4, mainly in BALB/c mice. In conclusion, the genetic background of the murine strain has a clear impact on the colonization ability of different H. suis strains and the immune response they evoke. A predominant Th17 response was observed, accompanied by a mild Th2 response, which is different from the Th17/Th1 response evoked by H. pylori infection.