Mouse granulocyte-macrophage colony-stimulating factor enhances viability of porcine embryos in defined culture conditions
Mouse granulocyte-macrophage colony-stimulating factor enhances viability of porcine embryos in defined culture conditions
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DOI：
10.1016/j.anireprosci.2003.11.005
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发表时间：
2004-08-01
影响因子：
2.2
通讯作者：
Kim, NH
中科院分区：
文献类型：
--
作者：
Cui, XS;Lee, JY;Kim, NH
The aim of this study was to determine the effects of mouse granulocyte-macrophage colony-stimulating factor (mGM-CSF) on development of porcine parthenotes and nuclear transferred embryos, and on their expression of implantation-related genes. In the presence of bovine serum albumin, mGM-CSF did not increase the percentage of oocytes that developed to the blastocyst stage and at day 7 did not increase cell numbers of embryos. Addition of 2 ng/ml GM-CSF to protein-free culture medium significantly increased the compaction and blastocoel formation of 1- to 2-cell parthenotes developing in vitro. However, total cell numbers were not increased when they were cultured in the presence of GM-CSF. Semi-quantitative reverse transcriptase polymerase chain reaction revealed that mGM-CSF enhances mRNA expression of the leukemia inhibitory factor receptor, but does not influence interleukin-6 or sodium/glucose co-transporter protein gene expression in blastocyst stage parthenotes. These results suggest that mGM-CSF may enhance viability of porcine embryos developing in vitro in a defined culture medium. (C) 2004 Elsevier B.V. All rights reserved.
