Comprehensive analysis of classical and newly described staphylococcal superantigenic toxin genes in Staphylococcus aureus isolates
Comprehensive analysis of classical and newly described staphylococcal superantigenic toxin genes in Staphylococcus aureus isolates
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DOI：
10.1016/j.femsle.2005.04.007
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发表时间：
2005-05-15
影响因子：
2.1
通讯作者：
Shinagawa, K
中科院分区：
文献类型：
--
作者：
Omoe, K;Hu, DL;Shinagawa, K
本文介绍了一种利用四套多重PCR技术对18种经典和新发现的葡萄球菌超抗原毒素基因进行检测的方法。对69株食物中毒金黄色葡萄球菌和97株健康人鼻拭子分离株进行超抗原毒素基因分型，共发现32种超抗原毒素基因型。金黄色葡萄球菌分离株携带多种毒素基因。毒素基因型和毒素基因编码谱的移动的遗传元件之间的关系分析表明，它可能在确定超抗原毒素基因型的S。金黄色葡萄球菌作为毒素基因编码移动的遗传元件的组合。(c)2005年，欧洲微生物学会联合会。Elsevier B.V.出版，保留所有权利。
We describe a comprehensive detection system for 18 kinds of classical and newly described staphylococcal superantigenic toxin genes using four sets of multiplex PCR. Superantigenic toxin genotyping of Staphylococcus aureus for 69 food poisoning isolates and 97 healthy human nasal swab isolates revealed 32 superantigenic toxin genotypes and showed that many S. aureus isolates harbored multiple toxin genes. Analysis of the relationship between toxin genotypes and toxin genes encoding profiles of mobile genetic elements suggests its possible role in determining superantigenic toxin genotypes in S. aureus as combinations of toxin gene-encoding mobile genetic elements. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.