Understanding RNA-Chromatin Interactions Using Chromatin Isolation by RNA Purification (ChIRP)
Understanding RNA-Chromatin Interactions Using Chromatin Isolation by RNA Purification (ChIRP)
复制标题
DOI：
10.1007/978-1-4939-6380-5_10
复制
发表时间：
2016-01-01
期刊：
影响因子：
--
通讯作者：
Chang, Howard Y.
中科院分区：
文献类型：
--
作者：
Chu, Ci;Chang, Howard Y.
ChIRP是一种研究长链非编码RNA（lncRNA）-染色质相互作用的新技术。RNA和染色质在体内使用甲醛或戊二醛交联，并使用与靶RNA杂交的生物素化反义寡核苷酸纯化。然后将共沉淀的DNA纯化并通过定量PCR（qPCR）或高通量测序进行分析。
ChIRP is a novel and easy-to-use technique for studying long noncoding RNA (lncRNA)-chromatin interactions. RNA and chromatin are cross-linked in vivo using formaldehyde or glutaraldehyde, and purified using biotinylated antisense oligonucleotides that hybridize to the target RNA. Co-precipitated DNA is then purified and analyzed by quantitative PCR (qPCR) or high-throughput sequencing.