Human adipose-derived stem cells isolated from young and elderly women: their differentiation potential and scaffold interaction during in vitro osteoblastic differentiation
Human adipose-derived stem cells isolated from young and elderly women: their differentiation potential and scaffold interaction during in vitro osteoblastic differentiation
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DOI：
10.3109/14653240903079393
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发表时间：
2009-01-01
期刊：
影响因子：
4.5
通讯作者：
Brini, Anna T.
中科院分区：
文献类型：
--
作者：
de Girolamo, Laura;Lopa, Silvia;Brini, Anna T.
背景aimsSeveral authors have demonstrated that adipose tissue contains multipotent cells capable of differentiating into several lineages，including bone，cartilage and fat.MethodsThis study compared human adipose derived stem cells（hASC）isolated from 26 female donors，under 35 and over 45 years old，showing different in their cell number and proliferation，并评估其在体外脂肪细胞和成骨细胞分化potential.ResultsThe细胞产量的hASC从老年人供体显着大于从年轻的供体，而他们的克隆形成的潜力似乎略有减少。从年轻女性和老年女性中分离的hASC在体外脂肪细胞分化方面没有显著差异，而成骨细胞的潜力显着降低。我们还评估了羟基磷灰石（HAP）和碳化硅（SiC-PECVD）对hASC的影响。即使在支架上培养，来自年轻供体的hASC也比来自老年供体的hASC更好地分化为成骨样细胞;它们的分化能力因HAP的存在而上调，而SiC-PECVD对hASC的成骨分化无明显影响。结论hASC大量存在于脂肪组织中，其分化特征表明其可用于成功的骨再生过程in vivo.我们已经表明，年龄似乎并不影响细胞活力和体外脂肪细胞分化显着，而它确实影响成骨细胞分化，在存在和不存在的二维和三维支架。
Background aimsSeveral authors have demonstrated that adipose tissue contains multipotent cells capable of differentiation into several lineages, including bone, cartilage and fat.MethodsThis study compared human adipose-derived stem cells (hASC) isolated from 26 female donors, under 35 and over 45 years old, showing differences in their cell numbers and proliferation, and evaluated their in vitro adipocytic and osteoblastic differentiation potential.ResultsThe cellular yield of hASC from older donors was significantly greater than that from younger donors, whereas their clonogenic potential appeared slightly reduced. There were no significant discrepancies between hASC isolated from young and elderly women regarding their in vitro adipocytic differentiation, whereas the osteoblastic potential was significantly reduced by aging. We also assessed the influence of hydroxyapatite (HAP) and silicon carbide (SiC-PECVD) on hASC. Even when cultured on scaffolds, hASC from younger donors had better differentiation into osteoblast-like cells than hASC from older donors; their differentiation ability was up-regulated by the presence of HAP, whereas SiC-PECVD produced no significant effect on hASC osteoblastic differentiation.ConclusionsThe large numbers of hASC resident in adipose tissue and their differentiation features suggest that they could be used for a successful bone regeneration process in vivo. We have shown that age does not seem to affect cell viability and in vitro adipocytic differentiation significantly, whereas it does affects osteoblastic differentiation, in the absence and presence of two-dimensional and three-dimensional scaffolds.