BONE EMBEDDING IN PURE METHYL-METHACRYLATE AT LOW-TEMPERATURE PRESERVES ENZYME-ACTIVITIES
BONE EMBEDDING IN PURE METHYL-METHACRYLATE AT LOW-TEMPERATURE PRESERVES ENZYME-ACTIVITIES
复制标题
DOI：
10.1016/s0065-1281(87)80012-0
复制
发表时间：
1987-01-01
影响因子：
2.5
通讯作者：
RIFFAT, G
中科院分区：
文献类型：
--
作者：
CHAPPARD, D;PALLE, S;RIFFAT, G
纯甲基丙烯酸甲酯（MMA），一种广泛用于未脱钙骨研究的包埋介质，在低温（4 ℃）下聚合。C）。MMA的制备通过一个新的纯化程序产生一个绝对无水和催化树脂。采用氧化还原体系过氧化苯甲酰/N，N-二甲基苯胺为MMA化学聚合提供自由基。由于该作用在-20 ℃下被抑制，因此在3天内实现块的完全渗透。聚合反应在+4 ℃下进行。C.该方法提供了适用于类骨质组织组织形态计量学分析的未脱钙骨切片，四环素骨标记抗酒石酸盐磷酸酶。酶组织化学被证明是可能的纯MMA包埋骨，当这种低温包埋被使用。
Pure Methyl Methacrylate (MMA), a widely used embedding medium for undecalcified bone studies, was polymerized at low temperature (4.degree. C). MMA was prepared by a new purification procedure yielding an absolutely anhydrous and catalysed resin. The redox system benzoyle peroxide/NN-Dimethylanilin was use as the catalyzer-initiator system providing free radicals for the MMA chemical polymerization. Since the action is inhibited at -20.degree.C, complete infiltration of blocks is achieved within 3 d. Polymerization took place at +4.degree. C. The method provides undecalcified bone section suitable for histomorphometric analysis of osteoid tissue, tetracycline bone labeling Tartrate Resistant Phosphatase. Enzyme histochemistry was shown to be possible in pure MMA embedded bones, when this low temperature embedding was used.