Ralstonia basilensis M91-3, a denitrifying soil bacterium capable of using s-triazines as nitrogen sources.
Ralstonia basilensis M91-3, a denitrifying soil bacterium capable of using s-triazines as nitrogen sources.
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DOI：
10.1139/w02-113
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发表时间：
2002-12
影响因子：
2.8
通讯作者：
D. M. Stamper;M. Radosevich;K. Hallberg;S. Traína;O. Tuovinen
中科院分区：
文献类型：
--
作者：
D. M. Stamper;M. Radosevich;K. Hallberg;S. Traína;O. Tuovinen
本研究的目的是表征能够矿化阿特拉津（2-氯-4- n -异丙基-6- n -乙基-s-三嗪）的土壤细菌M91-3的系统发育和表型特征。根据16S rRNA序列的99.5%同源性和各种化学分类资料，鉴定该分离物为巴西鼠拉尔斯顿菌。该分离物使用阿特拉津作为能量、碳和氮的唯一来源。它也可以使用其他几种s-三嗪作为氮源。在乙炔堵塞条件下对氧化亚氮进行气相色谱分析，证实了Ralstonia basilensis M91-3具有反硝化作用。
The purpose of this study was to characterize the phylogenetic and phenotypic traits of M91-3, a soil bacterium capable of mineralizing atrazine (2-chloro-4-N-isopropyl-6-N-ethyl-s-triazine). The isolate was identified as Ralstonia basilensis based on 99.5% homology of the 16S rRNA sequence and various chemotaxonomic data. The isolate used atrazine as the sole source of energy, carbon, and nitrogen. It could also use several other s-triazines as nitrogen sources. Ralstonia basilensis M91-3 was capable of denitrification, which was confirmed by gas chromatographic analysis of nitrous oxide under acetylene blockage conditions.