A novel method for the construction of genome wide transcriptome maps
A novel method for the construction of genome wide transcriptome maps
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DOI：
10.1046/j.1365-313x.2002.01345.x
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发表时间：
2002-07-01
期刊：
影响因子：
7.2
通讯作者：
Visser, RGF
中科院分区：
文献类型：
--
作者：
Brugmans, B;del Carmen, AF;Visser, RGF
基因表达谱分析通常用于显示特定组织、治疗或发育阶段的转录组。本文利用cDNA-AFLP技术对拟南芥和马铃薯分离群体表达的转录本进行了研究。因此，后代基因类型之间的遗传差异在转录组中表现为多态。我们表明，多态转录本可以用作遗传标记，并允许构建连锁图谱。结果图谱显示，与基因组标记相反，转录组衍生的标记并没有聚集在染色体的特定区域，而且cDNA-AFLP标记专门针对转录活跃的区域。用于定位的cDNAAFLP标记来源于转录本中的DNA多态，而不是表达调控上的差异。转录组标记相对于(匿名)基因组标记在遗传分析、标记辅助育种和基于图谱的克隆中的应用潜力很大。
Expression profiling by cDNA-AFLP is commonly used to display the transcriptome of a specific tissue, treatment or developmental stage. In this paper, cDNA-AFLP has been used to study transcripts expressed in segregating populations from Arabidopsis thaliana and potato (Solanum tuberosum ). The genetic differences between the offspring genotypes are thus visualized as polymorphisms in the transcriptome. We show that polymorphic transcripts can be used as genetic markers and allow the construction of a linkage map. The resulting map shows that, in contrast to genomic markers, the transcriptome-derived markers did not cluster in particular areas of the chromosome, and that cDNA-AFLP markers are targeted specifically to transcriptionally active regions. The cDNA-AFLP markers used in mapping are derived from DNA polymorphisms in transcripts, rather than differences in expression regulation. The high potential of transcriptome markers as opposed to (anonymous) genomic markers for applications in genetic analyses, marker-assisted breeding and map-based cloning is discussed.