SPARC-null Mice Exhibit Lower Intraocular Pressures
SPARC-null Mice Exhibit Lower Intraocular Pressures
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DOI：
10.1167/iovs.08-2489
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发表时间：
2009-08-01
影响因子：
4.4
通讯作者：
Rhee, Douglas J.
中科院分区：
文献类型：
--
作者：
Haddadin, Ramez I.;Oh, Dong-Jin;Rhee, Douglas J.
目的。SPARC是一种基质细胞蛋白，在包括小梁网和睫状体在内的重塑组织中高度表达。该研究的假设是SPARC有助于调节眼压(IOP)。方法测定C57BL/6x129SvJ野生型、SPARC缺陷型和杂合子小鼠的昼夜眼压。进行了荧光光度测量以评估水的周转率。通过组织学、超声生物显微镜和光学相干断层扫描测量中央角膜厚度(CCT)。结果：在白天，SPARC基因缺失小鼠(n=142，16.9+/-2.4 mm Hg)的平均眼压低于WT小鼠(n=104，19.9+/-2.9 mm Hg；P<；10(-12))和杂合子小鼠(n=38，19.3+/-2.5 mm Hg；P<；10(-4))。在夜间，与WT和杂合子小鼠相比，SPARC基因缺失的小鼠的眼压也有明显的增加。WT和SPARC基因缺失小鼠之间的CCT没有显著差异。杂合子小鼠的角膜往往较厚(3.4%)。荧光光度测量表明，SPARC基因缺失小鼠的房水周转率与WT小鼠相同，如果不高于WT小鼠的话。SPARC缺失的虹膜角膜角度的光镜观察显示的形态与WT.结论：SPARC缺失的小鼠的眼压比具有相同CCT的WT对应的小鼠低。水的周转速度表明，其机理是增强了流出阻力。(投资眼科VS科学。网址：10.1167/iovs.08-2489
PURPOSE. SPARC is a matricellular protein that is highly expressed in remodeling tissues, including the trabecular meshwork and ciliary body. The hypothesis for the study was that SPARC contributes to the regulation of intraocular pressure (IOP). The IOPs of SPARC-null mice, their corresponding wildtype (WT), and heterozygous animals were compared.METHODS. Diurnal and nocturnal IOPs of C57Bl/6x129SvJ WT, SPARC-null, and heterozygous mice were measured. Fluoro-photometric measurements were made to assess aqueous turnover. Central corneal thickness (CCT) was measured using histology, ultrasound biomicroscopy, and optical coherence tomography. Iridocorneal angles were examined using light microscopy (LM).RESULTS. During the day, the mean IOP of SPARC-null mice (n = 142, 16.9 +/- 2.4 mm Hg) was lower than that of both WT mice (n = 104, 19.9 +/- 2.9 mm Hg; P < 10(-12)), and heterozygotes (n = 38, 19.3 +/- 2.5 mm Hg; P < 10(-4)). At night, SPARC-null mice also exhibited a blunted increase in IOP in comparison to WT and heterozygous mice. CCTs were not significantly different between WT and SPARC-null mice. Heterozygous mice tended to have thicker corneas (3.4%). Fluoro-photometric measurements suggest that aqueous turnover rates in SPARC-null mice are equal to if not greater than rates in WT mice. LM of the SPARC-null iridocorneal angle revealed morphology that is indistinguishable from WT.CONCLUSIONS. SPARC-null mice have lower IOPs than do their WT counterparts with equal CCTs. The rate of aqueous turnover suggests that the mechanism is enhanced outflow resistance. (Invest Ophthalmol Vis Sci. 2009; 50: 3771-3777) DOI: 10.1167/iovs.08-2489