Peculiarities of two different IAA binding sites functioning in kidney bean hypocotyl cell plasmalemma
Peculiarities of two different IAA binding sites functioning in kidney bean hypocotyl cell plasmalemma
复制标题
芸豆下胚轴细胞质膜中两个不同 IAA 结合位点的特性
DOI：
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
N. Anisimovienė
中科院分区：
文献类型：
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作者：
M. Jodinskienė;N. Anisimovienė
在通过延伸响应 IAA 的细胞质膜中发现了两个不同的 IAA 结合位点（最佳 pH 5.5 和 7.5）。第一个位点的特征是 IAA 受体，通过延伸介导细胞对 IAA 的反应。它具有 IAA 结合位点的结构 – /-H-RH-S-C-E-/。该位点在不通过伸长响应 IAA 的下胚轴细胞中不起作用。第二个位点（最佳 pH 7.5）的活性取决于 IAA 转运。根据该位点的特点，可以提出以下建议：该位点有1个组氨酸残基和含有-SH基的氨基酸在起作用； IAA 流入抑制剂 3-氯-4-羟基苯乙酸 (CHPAA) 能够与 IAA 的结合位点进行非常活跃的竞争：它可以将 IAA-ABP 相互作用减少 92%。我们的结果证实了几种不同的 ABP 可能在细胞中发挥作用，并且对于每种单独的反应可能需要单独的受体。
Two different IAA binding sites (optimal pH 5.5 and 7.5) were discovered in the plasmalemma of cells responding to IAA by elongation. The first site is characterized as IAA-receptor mediating cell response to IAA by elongation. It has a structure of the IAA binding site – /-H-RH-S-C-E-/. This site is not functioning in hypocotyl cells not responding to IAA by elongation. The activity of the second site (optimal pH 7.5) is dependent on IAA transport. Basing on the characteristics of this site, the following suggestions may be made: one residue of histidine and amino acid(s) containing the -SHgroup are functioning in this site; the IAA influx inhibitor 3-chloro-4-hydroxyphenylacetic acid (CHPAA) is capable of a very active competition with IAA in the binding site: it reduces IAA–ABP interaction by 92%. Our results confirmed that several different ABP may be functioning in the cell and that for each separate response an individual receptor may be required.