Retrospective study of 338 canine oral melanomas with clinical, histologic, and immunohistochemical review of 129 cases
Retrospective study of 338 canine oral melanomas with clinical, histologic, and immunohistochemical review of 129 cases
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DOI：
10.1354/vp.37-6-597
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发表时间：
2000-11-01
影响因子：
2.4
通讯作者：
Kottler, SJ
中科院分区：
文献类型：
--
作者：
Ramos-Vara, JA;Beissenherz, ME;Kottler, SJ
回顾了兽医诊断实验室（1992-1999）收到的338只犬的338只犬口腔黑色素瘤的诊断记录。在这些肿瘤中，选择122例加上另外7例来源不明的转移性黑色素瘤进行临床随访、组织学检查和免疫组化。松狮犬、金毛寻回犬和北京犬/贵宾犬混合品种的代表性过高，而义和团犬和德国牧羊犬品种的代表性不足。没有性别倾向，平均就诊年龄为11.4岁。49只犬因复发或转移而被人道处死。术后平均生存173天。牙龈和唇粘膜是最常见的部位。肿瘤由多角形细胞（27例，20.9%）、梭形细胞（44例，34.1%）或两者混合（多角形和梭形）（54例，41.9%）组成。透明细胞型（3例，2.3%）和腺样/乳头型（1例，0.8%）少见。6/6例口腔恶性黑色素瘤转移灶的形态学和免疫组化特征与原发灶相似。免疫组化检测，Melan A在113/122（92.6%）和5/7（71.9%）的口腔转移性黑色素瘤中检出。仅4/163例非黑素细胞肿瘤呈局灶性和弱阳性。抗波形蛋白、S100蛋白和神经元特异性烯醇化酶抗体分别对129例（100%）、98例（76%）和115例（89.1%）黑色素瘤染色。针对其他黑素细胞相关抗原（酪氨酸酶，糖蛋白100）的抗体没有产生足够的染色。我们得出结论，Melan A是犬黑色素瘤的特异性和敏感性标志物。
Diagnostic records from 338 canine oral melanomas in 338 dogs received at the Veterinary Medical Diagnostic Laboratory (1992-1999) were reviewed. Of these tumors, 122 plus an additional 7 metastatic melanomas of unknown origin were selected for clinical follow-up, histologic review, and immunohistochemistry. Chow Chow, Golden Retriever, and Pekingese/Poodle mix breeds were overrepresented, whereas Boxer and German Shepherd breeds were underrepresented. There was no gender predisposition and the average age at presentation was 11.4 years. Forty-nine dogs were euthanized due to recurrence or metastasis. The average postsurgical survival time was 173 days. The gingiva and the labial mucosa were the most common sites. Most tumors were composed of either polygonal cells (27 cases, 20.9%), spindle cells (44 cases, 34.1%), or a mixture of the two (polygonal and spindle) (54 cases, 41.9%). Clear cell (3 cases, 2.3%) and adenoid/papillary (1 case, 0.8%) patterns were uncommon. The metastases of 6/6 oral melanomas had morphologic and immunohistochemical features similar to those of the primary tumors. Immunohistochemically, Melan A was detected in 113/122 oral (92.6%) and 5/7 (71.9%) metastatic melanomas. Only 4/163 nonmelanocytic tumors were focally and weakly positive for Melan A. Antibodies against vimentin, S100 protein, and neuron-specific enolase stained 129 (100%), 98 (76%), and 115 (89.1%) of 129 melanomas, respectively. Antibodies against other melanocytic-associated antigens (tyrosinase, glycoprotein 100) did not yield adequate staining. We conclude that Melan A is a specific and sensitive marker for canine melanomas.