Stress-induced changes in sleep and associated neuronal activity in rat hippocampus and amygdala
Stress-induced changes in sleep and associated neuronal activity in rat hippocampus and amygdala
复制标题
DOI：
10.1016/j.neuroscience.2008.01.042
复制
发表时间：
2008-04-22
期刊：
影响因子：
3.3
通讯作者：
Laxmi, T. R.
中科院分区：
文献类型：
--
作者：
Hegde, P.;Singh, K.;Laxmi, T. R.
压力会增加焦虑和抑郁的易感性。通过对快速眼动(REM)睡眠状态下雄性Wistar大鼠脑电(EEG)的测定，研究了急性束缚应激对大鼠杏仁核-海马环路的影响。电极被立体定向植入海马区(海马区CA1和CA3区)和杏仁核(外侧核)。在应激之前，进行了两次基线记录。24小时后，将大鼠暴露于一次急性束缚应激(AIS)，持续2小时，在释放后和随后的几天，通过与基线测量相比较，分析在REM睡眠中因应激而发生的电生理变化。我们的结果表明，急性束缚应激在暴露后的前24小时内显著增加了REM睡眠。除了睡眠模式的变化，我们还观察到海马区CA1区的theta振荡增加，海马区和杏仁核之间的theta范围(4-8赫兹)的一致性降低。这些结果表明，单一暴露于令人厌恶的经历，如束缚应激，可以导致神经元活动的动态变化，并改变睡眠形态。在本研究中获得的结果与人类因创伤后应激障碍(PTSD)而遭受恐慌和焦虑的患者的结果相当。(C)2008年IBRO。爱思唯尔有限公司出版。保留所有权利。
Stress increases vulnerability to anxiety and depression. We have investigated the effect of acute immobilization stress in amygdalohippocampal circuits by measuring the electroencephalogram (EEG) in male Wistar rats during rapid eye movement (REM) sleep. Electrodes were implanted stereotaxically in the hippocampus (CA1 and CA3 subregions of the hippocampus) and the amygdala (lateral nucleus). Prior to the stress, two baseline recordings were taken. Twenty-four hours later rats were exposed once to acute immobilization stress (AIS) session for 2 h. After the release and on subsequent days, electrophysiological changes that occurred due to stress during REM sleep were analyzed by comparing them with baseline measurements. Our results suggest that acute immobilization stress induced significant increase in REM sleep in the first 24 h after the exposure. In addition to changes in the sleep patterns, we have observed increased theta oscillations in CA1 area of the hippocampus with decreased coherence at theta range (4-8 Hz) between hippocampus and amygdala. These results suggest that single exposure to aversive experience such as immobilization stress can lead to dynamic changes in neuronal activities with altered sleep morphology. The results obtained in the present study are comparable to those seen in human patients suffering from panic, and anxiety due to posttraumatic stress disorder (PTSD). (C) 2008 IBRO. Published by Elsevier Ltd. All rights reserved.