Assessment of enzyme induction and aerenchyma formation as mechanisms for flooding tolerance in Trifolium subterraneum 'Park'
Assessment of enzyme induction and aerenchyma formation as mechanisms for flooding tolerance in Trifolium subterraneum 'Park'
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DOI：
10.1093/aob/mcf022
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发表时间：
2003-01-01
期刊：
影响因子：
4.2
通讯作者：
Saglio, P
中科院分区：
文献类型：
--
作者：
Aschi-Smith, S;Cha誰bi, W;Saglio, P
本研究的目的是评价酶诱导和通风组织的形成在延长对土壤淹水的耐受性中的作用。苗木在水培容器中生长，最初通气3周，然后在不通气的情况下最多3周。在不曝气的情况下，培养1h后，水培介质中的氧浓度下降到1.5%。在极度缺氧的3周内，原生根死亡，取而代之的是大量不定根。不定根中多种糖酵解酶和发酵酶活性增加。切除的不定根能够在没有乳酸产生的情况下立即诱导乙醇，这与能量负荷有关，高于有氧维持对照的切除根。在大车前的不定根中测得的能荷更高。有趣的是，血红蛋白可能与能量电荷相关。纵横切面光学显微镜下，不定根内可见气孔组织。我们认为，通过透气组织避免根的缺氧是红三叶‘公园’延长根耐性的主要机制。(C)2003年《植物学公司年鉴》。
The objective of this study was to evaluate the role of enzyme induction and aerenchyma formation in prolonged tolerance to soil flooding in a variety of underground clover (Trifolium subterraneum 'Park') previously selected for resistance. Seedlings were grown in hydroponic tanks, initially with aeration for 3 weeks and subsequently in the absence of aeration for up to 3 weeks. After 1 h in the absence of aeration, the oxygen concentration in the hydroponic medium had decreased to 1.5 %. During the 3 weeks of extreme oxygen deficiency, primary roots died and were replaced by considerable numbers of adventitious roots. Activities of many glycolytic and fermentative enzymes increased in adventitious roots. Excised adventitious roots were capable of immediate induction of ethanol in the absence of lactate production, in association with energy charge higher than that in excised roots of aerobically maintained controls. Energy charge was even higher when measured in adventitious roots in planta. Interestingly, haemoglobin protein could be correlated with energy charge. Aerenchyma was readily visualized in adventitious roots by optical microscopy of longitudinal and transverse sections. We conclude that avoidance of root anoxia via aerenchyma is the major mechanism for prolonged root tolerance in Trifolium subterraneum 'Park'. (C) 2003 Annals of Botany Company.