New peptidomimetic polymers for antifouling surfaces
New peptidomimetic polymers for antifouling surfaces
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DOI：
10.1021/ja0522534
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发表时间：
2005-06-08
影响因子：
15
通讯作者：
Messersmith, PB
中科院分区：
文献类型：
--
作者：
Statz, AR;Meagher, RJ;Messersmith, PB
将治疗和诊断医疗器械暴露于生物液体通常伴随着蛋白质、细胞和微生物的界面吸附。表面的生物污染可能导致器械性能受损或成本增加，并且在某些情况下可能危及患者的生命。尽管许多聚合物涂层在防止蛋白质和细胞吸附在表面上方面取得了短期成功，但没有一种被证明是赋予长期生物污垢抗性的理想选择。在这里，我们描述了一种新的仿生防污N-取代甘氨酸聚合物（类肽）含有一个C-末端肽锚来自贻贝粘附蛋白中发现的残基的聚合物到表面上的强大的附着。选择聚合物的类肽部分的甲氧基乙基侧链是因为其化学上与已知的聚乙二醇（PEG）的重复单元相似，而选择5-mer锚定肽的组成以直接模拟已知的贻贝粘附蛋白的富含DOPA和Lys的序列。在体外试验中，用这种仿生肽-类肽缀合物修饰的表面显示出血清蛋白吸附的显著降低和对哺乳动物细胞附着的抵抗力超过5个月。这些新的基于合成肽的可降解聚合物可以提供对生理、海洋和工业环境中的表面生物污染的长期控制。
Exposure of therapeutic and diagnostic medical devices to biological fluids is often accompanied by interfacial adsorption of proteins, cells, and microorganisms. Biofouling of surfaces can lead to compromised device performance or increased cost and in some cases may be life-threatening to the patient. Although numerous antifouling polymer coatings have enjoyed short-term success in preventing protein and cell adsorption on surfaces, none have proven ideal for conferring long-term biofouling resistance. Here we describe a new biomimetic antifoulingN-substituted glycine polymer (peptoid) containing aC-terminal peptide anchor derived from residues found in mussel adhesive proteins for robust attachment of the polymer onto surfaces. The methoxyethyl side chain of the peptoid portion of the polymer was chosen for its chemical resemblance to the repeat unit of the known antifouling polymer poly(ethylene glycol) (PEG), whereas the composition of the 5-mer anchoring peptide was chosen to directly mimic the DOPA- and Lys-rich sequence of a known mussel adhesive protein. Surfaces modified with this biomimetic peptide−peptoid conjugate exhibited dramatic reduction of serum protein adsorption and resistance to mammalian cell attachment for over 5 months in an in vitro assay. These new synthetic peptide based antifouling polymers may provide long-term control of surface biofouling in the physiologic, marine, and industrial environments.