Effectiveness of beneficial bacteria to promote systemic resistance of grapevine to gray mold as related to phytoalexin production in vineyards
Effectiveness of beneficial bacteria to promote systemic resistance of grapevine to gray mold as related to phytoalexin production in vineyards
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DOI：
10.1007/s11104-015-2783-z
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发表时间：
2016-08-01
期刊：
影响因子：
4.9
通讯作者：
Trotel-Aziz, Patricia
中科院分区：
文献类型：
--
作者：
Aziz, Aziz;Verhagen, Bas;Trotel-Aziz, Patricia
根际枯草芽孢杆菌PTA-271（271）和内生荧光假单胞菌PTA-CT 2（CT 2）和成团泛菌PTA-AF 2（AF 2）细菌能够诱导葡萄藤对B的系统抗性（ISR）。cinerea，但ISR标记和它们的成本仍然未知的葡萄园。在这项研究中，我们调查了单一和二元组合的选择细菌诱导ISR的有效性之间的关系，并能够触发植物抗毒素积累，作为一个潜在的标志，抗病性，在叶片和浆果，以及它们对葡萄产量的影响在葡萄园。葡萄藤植物在2006年期间处理在两个葡萄园的淋水土壤与单一或二元混合物的细菌。诱导抗B。评价了灰葡萄属植物，并通过HPLC分析了叶和浆果中的芪类植物抗毒素。葡萄产量也通过成熟时葡萄丛的数量和重量来评估。单一细菌和混合细菌都能有效降低葡萄园叶片和浆果中灰霉病的严重程度。疾病控制伴随着显着积累的芪类植物抗毒素，反式白藜芦醇和ε-葡萄糖苷，在细菌化的植物的叶子和浆果。δ-葡萄糖苷也积累，但仅在处理植物的浆果中。与AF 2和271相比，在用单一CT 2处理的植物中，疾病症状的减少和白藜芦醇和葡萄糖苷的积累更高。用这些分离物的二元混合物处理葡萄藤植物导致在叶中CT 2 + AF 2和在浆果中CT 2 +271的显著性能。这些结果揭示了CT 2单独或与AF 2或与271组合在引发葡萄藤对B的抗性中的功效。在不影响葡萄产量的情况下，提高白藜芦醇和葡萄糖苷的系统积累。
The rhizospheric Bacillus subtilis PTA-271 (271) and endophytic Pseudomonas fluorescens PTA-CT2 (CT2) and Pantoea agglomerans PTA-AF2 (AF2) bacteria are able to induce systemic resistance (ISR) in grapevine against B. cinerea, but ISR markers and their costs remained unknown in vineyards. In this study, we investigated the relationship between the effectiveness of single and binary combinations of selected bacteria to induce ISR and their ability to trigger phytoalexin accumulation, as a potential marker for disease resistance, in leaves and berries, as well as their impact on grape yield in vineyards.Grapevine plants were treated during 2006 in two vineyards by drenching soil with single or binary mixtures of bacteria. Induced resistance against B. cinerea was evaluated and stilbenic phytoalexins were analyzed by HPLC in both leaves and berries. Grape yield was also assessed as number and weight of clusters at ripening.Both single and mixtures of bacteria were effective in reducing gray mold severity in the leaves and berries in vineyards. Disease control was accompanied by a significant accumulation of stilbenic phytoalexins, trans-resveratrol and epsilon-viniferin, in both leaves and berries in the bacterized plants. delta-Viniferin also accumulated, but only in berries of the treated plants. Reduction of disease symptoms and accumulation of resveratrol and viniferins were higher in the plants treated with single CT2 compared to AF2 and 271. Treatment of grapevine plants with binary mixtures of these isolates resulted in a significant performance of CT2+AF2 in leaves and CT2+271 in berries. On the other hand, bacterial treatments did not show any negative effect on grape yield.These results revealed the efficacy of CT2 alone or in combination with AF2 or with 271 in triggering grapevine resistance against B. cinerea and enhancing systemic accumulation of resveratrol and viniferins, without compromising grape yield.