The association of amyloid P-component (AP) with the amyloid fibril: an updated method for amyloid fibril protein isolation.
The association of amyloid P-component (AP) with the amyloid fibril: an updated method for amyloid fibril protein isolation.
复制标题
淀粉样蛋白 P 成分 (AP) 与淀粉样原纤维的关联：淀粉样原纤维蛋白分离的更新方法。
DOI：
10.1080/10826068208070597
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Deal,CL
中科院分区：
文献类型：
--
作者：
Skinner,M;Shirahama,T;Cohen,AS;Deal,CL
提出了一种淀粉样蛋白原纤维分离方法，该方法使用柠檬酸盐以及盐水洗涤来从淀粉样蛋白原纤维中解离淀粉样蛋白P-组分（AP）的钙依赖性键。在两个富含淀粉样蛋白的组织中，在每次盐水和柠檬酸盐洗涤中定量AP的量，并且总共分离出13.8%和20.8%的淀粉样蛋白原纤维。从这些和另外22个富含淀粉样蛋白的组织中去除的AP的量比以前认识到的要大。AP蛋白仅在存在淀粉样蛋白纤维的组织中存在。在正常的非淀粉样组织中不能发现，在去除纤维后的组织沉积物中也不能发现。
An amyloid fibril isolation procedure is proposed which uses citrate as well as saline washes to dissociate the calcium dependent linkage of amyloid P-component (AP) from the amyloid fibril. In two amyloid rich tissues, the amount of AP was quantitated in each saline and citrate wash and totalled 13.8% and 20.8% of the amyloid fibrils isolated. The amount of AP removed from these and 22 additional amyloid rich tissues was greater than had previously been recognized.AP protein was present in tissue only when amyloid fibrils were present. It could not be found in normal non-amyloidotic tissue, nor could it be found in tissue sediment after the fibrils were removed.