Sequence structure and population data of two X-linked markers: DXS7423 and DXS8377
Sequence structure and population data of two X-linked markers: DXS7423 and DXS8377
复制标题
两个 X 连锁标记 DXS7423 和 DXS8377 的序列结构和群体数据
DOI：
10.1007/s00414-002-0316-9
复制
发表时间：
2002
影响因子：
2.1
通讯作者：
J. Riancho
中科院分区：
文献类型：
--
作者：
M. T. Zarrabeitia;T. Amigo;C. Sañudo;M. D. de Pancorbo;J. Riancho
摘要。DXS7423和DXS8377是位于x染色体q28带的两个微卫星标记，我们开发了一个单反应扩增这两个标记的方案，测序了最常见的等位基因，并研究了等位基因频率在西班牙人群样本中。DXS7423等位基因的变异是由TCCA重复数不同引起的，有5个等位基因的表观大小在181 ~ 197 bp之间。女性样本的歧视概率（PD）为87%，排除概率（PE）为71%。DXS8377是一个高度多态性的标记，（CTC）、（TCC）和（TTC）重复次数不同。共发现18个大小不等的等位基因（204 ~ 258 bp）， PD和PE分别为99%和93%。这些数据表明DXS7423和DXS8377可以成为非常有用的遗传法医研究标记。
Abstract. DXS7423 and DXS8377 are two microsatellite markers located in the q28 band of chromosome X. We developed a protocol to amplify both markers in a single reaction, sequenced the most common alleles and studied allele frequencies in a Spanish population sample. DXS7423 allele variability was due to different numbers of (TCCA) repeats and five different alleles were found with apparent sizes between 181 and 197 bp. The probability of discrimination (PD) was 87% for female samples, and the expected probability of exclusion (PE) was 71%. DXS8377 appeared as a highly polymorphic marker with variable numbers of (CTC), (TCC) and (TTC) repeats. We found 18 alleles of different sizes (204–258 bp) and the PD and PE were 99% and 93%, respectively. These data suggest that DXS7423 and DXS8377 can be very useful markers for genetic forensic studies.