Adsorption of polyethyleneimine characterized by spectroscopic ellipsometry
Adsorption of polyethyleneimine characterized by spectroscopic ellipsometry
复制标题
DOI：
10.1080/10020070512330008
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Xiubo Zhao;F. Pan;J. Lu
中科院分区：
文献类型：
--
作者：
Xiubo Zhao;F. Pan;J. Lu
摘要聚乙烯亚胺（PEI）是一种基因转染试剂，近二十年来得到了广泛的研究。在这项工作中，我们通过检查其在亲水性氧化硅/水界面的界面吸收及其在生理条件下固定生物活性DNA的能力来探索其物理化学性质。发现其表面过量随着pH在pH 3和pH 10之间而逐渐增加。在pH值为7时，表面吸附量随盐浓度的增加而增加。发现在这些生理条件下，固定化的DNA的量由PEI和DNA之间的电荷比控制。因此，可以通过调节PEI的预吸附量来调节固定的生物活性DNA的量。该研究为通过界面吸附制备生物传感器和基因芯片提供了有用的信息。由Biocompatibles UK和EPSRC支持。
Abstract Polyethyleneimine (PEI) is a well known gene transfection reagent that has been extensively studied in the pass two decades. In this work, we have explored its physiochemical properties by examining its interfacial absorption at the hydrophilic silicon oxide/water interface and its ability to immobilize bioactive DNA under physiological conditions. Its surface excess was found to show a gradual increase with pH between pH 3 and pH 10. At pH 7 the surface absorbed amount increased with increasing salt concentaration. The amount of DNA immobilzed was found to be controlled by the charge ratio between PEI and DNA under these physiological conditions. Thus, the amount of bioactive DNA immobilized can be tuned by badjusting the amount of PEI preadsorbed. This study provides useful information for fabrication of biosensors and gene chips via interfacial adsorption. Supported by Biocompatibles UK and EPSRC.