The CD40 ligand directly activates T-lymphocytes via tyrosine phosphorylation dependent PKC activation
The CD40 ligand directly activates T-lymphocytes via tyrosine phosphorylation dependent PKC activation
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DOI：
10.1006/bbrc.1997.7415
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发表时间：
1997-10-09
影响因子：
3.1
通讯作者：
Gulbins, E
中科院分区：
文献类型：
--
作者：
Brenner, B;Koppenhoefer, U;Gulbins, E
B 淋巴细胞的激活主要取决于 CD40 受体与其配体的相互作用。在这里，我们提供的证据表明 CD40 配体 (CD40L) 也可作为 T 淋巴细胞的直接刺激分子。通过 CD40L 激活 T 淋巴细胞会诱导细胞蛋白（包括 PLC gamma）酪氨酸磷酸化。 PLC gamma 的酪氨酸磷酸化与 IP3 和 Ca2+ 释放以及 PKC 激活相关。 Herbimycin A 对 src 样酪氨酸激酶的抑制可防止这些激活事件，表明酪氨酸磷酸化在通过 CD40L 激活 T 淋巴细胞中发挥着至关重要的作用。 (C) 1997 年学术出版社。
The activation of B-lymphocytes depends critically on the interaction of the CD40 receptor with its ligand. Here, we provide evidence that the CD40 ligand (CD40L) also functions as a direct stimulatory molecule for T-lymphocytes. Activation of T-lymphocytes via CD40L induces tyrosine phosphorylation of cellular proteins including PLC gamma. Tyrosine phosphorylation of PLC gamma correlates with an IP3- and Ca2+-release and an activation of PKC. Inhibition of src-like tyrosine kinases by Herbimycin A prevents these activation events suggesting a crucial role of tyrosine phosphorylation in T-lymphocyte activation via CD40L. (C) 1997 Academic Press.