Elevated expression of TGIF is involved in lung carcinogenesis
Elevated expression of TGIF is involved in lung carcinogenesis
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TGIF表达升高参与肺癌发生
DOI：
10.1007/s13277-015-3615-8
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发表时间：
2015-12-01
期刊：
影响因子：
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通讯作者：
Zhang, Congke
中科院分区：
文献类型：
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作者：
Wang, Yadong;Wang, Haiyu;Zhang, Congke
本研究旨在探讨甘油三酯相互作用因子(TGIF)在肺癌发生发展中的表达。用苯并(A)芘(BaP)诱导人支气管上皮(16HBE)细胞恶性转化。采用软琼脂实验和裸鼠成瘤实验。用BaP进行体内成瘤实验。用Western blotting、免疫组织化学和定量聚合酶链式反应检测TGIF的表达。我们观察到肺癌组织中TGIF信使RNA的表达水平高于癌旁组织。我们观察到A549和H1299细胞系的TGIF mRNA和蛋白水平明显高于16HBE细胞系。与溶剂对照组相比，BaP诱导的16HBE细胞TGIF蛋白和mRNA表达增加。我们观察到16HBE-bap细胞的TGIF mRNA和蛋白水平显著高于16HBE对照细胞。我们观察到BaP组小鼠肺组织中TGIF基因和蛋白的表达水平明显高于对照组。我们的结果提示TGIF的高表达参与了肺癌的发生。
The purpose of this study was to explore the expression of TG-interacting factor (TGIF) in lung carcinogenesis. Malignant transformation of human bronchial epithelial (16HBE) cell was established by benzo(a)pyrene (BaP) treatment. Soft agar assay and tumor formation assay in nude mice were applied. Tumorigenesis experiment in vivo was done by BaP treatment. Western blotting, immunohistochemistry, and quantitative polymerase chain reaction were used to detect TGIF expression. We observed a higher level of TGIF messenger RNA (mRNA) in lung cancer tissues than that in paracancerous tissues. We observed significantly higher levels of TGIF mRNA and protein in A549 and H1299 cell lines than that in 16HBE cell. Increased expressions of TGIF protein and mRNA were observed in 16HBE cells induced by BaP treatment as compared to those in solvent control group. We observed significantly higher levels of TGIF mRNA and protein in 16HBE-BaP cells than that in 16HBE-control cells. We observed significantly higher levels of TGIF mRNA and protein in mice lung tissues treated with BaP than that in control group. Our results suggested that elevated expression of TGIF was involved in lung carcinogenesis.