ICEFormat-the image cytometry experiment format.
ICEFormat-the image cytometry experiment format.
复制标题
ICEFormat-图像细胞计数实验格式。
DOI：
10.1002/cyto.a.22212
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Novo,David
中科院分区：
文献类型：
--
作者：
Spidlen,Josef;Novo,David
在图像细胞术中，大多数仪器以专有文件格式生成图像和特征数据，这些文件格式与特定硬件制造商提供的分析软件紧密绑定。获取这些格式的规范可能是有问题的，因此，第三方软件应用程序很难甚至不可能处理这些格式。因此，来自不同仪器的数据融合和超出制造商提供的软件能力的进一步处理几乎是不可能的，这阻碍了实验结果的互操作性和独立验证。这个问题已经得到了社会各界的广泛认可，这导致了提高二进制图像数据的可读性的努力。1990年，国际细胞学促进会(ISAC)提出了一种图像细胞学数据文件(1)的文件格式，其中包括存储二进制图像文件、关于图像的基本元数据以及底层实验。其他几个团体也推出了根据其特定需求量身定做的规格。此外，已经开发了数十种图像文件格式用于一般图像交换目的。其中许多是目前被常用的图像处理软件工具广泛使用的。在临床和病理环境中，数字图像标准化工作由国家电气制造商协会(NEMA)协调，该协会管理医学数字成像和通信(DICOM)(2)规范。虽然DICOM是处理、存储和传输医学成像信息的标准，但它包含了数字显微镜和图像细胞术中使用的数十种文件格式
IN image cytometry, most instruments produce image and feature data in proprietary file formats that are tightly bound to analytical software provided by the specific hardware manufacture. Obtaining specifications for these formats can be problematic and as such, these formats are difficult or even impossible to process by third party software applications. Consequently, amalgamating data from different instruments and further processing beyond the capabilities of the software provided by the manufacturer is nearly impossible, which hinders interoperability and independent validation of experimental results. This issue has been widely recognized by the community, which resulted in efforts to increase the readability of binary image data.In 1990, the International Society for Advancement of Cytometry (ISAC) proposed a file format for image cytometry data files (1), which included storing binary image files, basic metadata about images as well as underlying experiments. Several other groups also introduced specifications tailored to their particular needs. In addition, several dozens of image file formats have been developed for general image interchange purposes. Many of these are currently widely used by common image processing software tools. In the clinical and pathology environment, digital image standardization efforts are coordinated by the National Electrical Manufacturers Association (NEMA), which manages the Digital Imaging and Communications in Medicine (DICOM)(2) specification. While DICOM is a standard for handling, storing, and transmitting medical imaging information, it incorporates dozens of file formats used in digital microscopy and image cytometry in