Expression analysis of the novel gene collagen triple helix repeat containing-1 (Cthrc1)
Expression analysis of the novel gene collagen triple helix repeat containing-1 (Cthrc1)
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DOI：
10.1016/j.modgep.2006.03.008
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发表时间：
2006-10-01
影响因子：
1.2
通讯作者：
Lindner, Volkhard
中科院分区：
文献类型：
--
作者：
Durmus, Tahir;LeClair, Renee J.;Lindner, Volkhard
我们最近发现胶原三螺旋重复包含-1 （Cthrc1）是动脉损伤后内皮成纤维细胞诱导的新基因。Cthrc1是一种30 kDa的分泌蛋白，具有抑制胶原基质合成的能力。Cthrc1也被糖基化，并保留与Cthrc1在细胞外空间存在一致的信号序列。在受伤的动脉和皮肤伤口中，我们发现Cthrc1的表达与肌成纤维细胞和胶原基质沉积部位有关。此外，我们证明了Cthrc1在体外抑制胶原基质沉积。利用原位杂交和免疫组织化学技术，我们在小鼠胚胎发育和出生后组织中表征了Cthrc1的表达域。在小鼠胚胎中，Cthrc1在内脏内胚层、脊索、神经管、发育中的肾脏和心脏中表达。在发育中的骨骼中，即软骨原基、生长板软骨（不含肥厚带）、骨基质和骨膜中，观察到大量表达Cthrc1。成人骨仅在骨基质和骨膜中表达Cthrc1，而关节软骨缺乏表达。Cthrc1通常在上皮-间质界面表达，包括表皮和真皮、角膜基底上皮、气道上皮、食管上皮、脉络膜丛上皮和脑膜。在成人肾脏中，收集管和远端小管表达Cthrc1。总的来说，Cthrc1的表达位点与tgf - β家族成员和间质性胶原的表达位点有很大的重叠。本研究为了解Cthrc1的潜在功能提供了有用的信息。(c) 2006 Elsevier B.V.版权所有
We recently identified collagen triple helix repeat containing-1 (Cthrc1) as a novel gene induced in adventitial fibroblasts after arterial injury. Cthrc1 is a 30 kDa secreted protein that has the ability to inhibit collagen matrix synthesis. Cthrc1 is also glycosylated and retains a signal sequence consistent with the presence of Cthrc1 in the extracellular space. In injured arteries and skin wounds, we have found Cthrc1 expression to be associated with myofibroblasts and sites of collagen matrix deposition. Furthermore, we demonstrated that Cthrc1 inhibits collagen matrix deposition in vitro. Using in situ hybridization and immunohistochemistry, we characterized the expression domains of Cthrc1 during murine embryonic development and in postnatal tissues. In mouse embryos, Cthrc1 was expressed in the visceral endoderm, notochord, neural tube, developing kidney, and heart. Abundant expression of Cthrc1 was observed in the developing skeleton, i.e., in cartilage primordia, in growth plate cartilage with exclusion of the hypertrophic zone, in the bone matrix and periostium. Bones from adults showed expression of Cthrc1 only in the bone matrix and periostium while the articular cartilage lacked expression. Cthrc1 is typically expressed at epithelial-mesenchymal interfaces that include the epidermis and dermis, basal corneal epithelium, airway epithelium, esophagus epithelium, choroid plexus epithelium, and meninges. In the adult kidney, collecting ducts and distal tubuli expressed Cthrc1. Collectively, the sites of Cthrc1 expression overlap considerably with those reported for TGF-beta family members and interstitial collagens. The present study provides useful information towards the understanding of potential Cthrc1 functions. (c) 2006 Elsevier B.V. All rights reserved.