Synchronised cycling gene oscillations in presomitic mesoderm cells require cell-cell contact
Synchronised cycling gene oscillations in presomitic mesoderm cells require cell-cell contact
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DOI：
10.1387/ijdb.041958mm
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发表时间：
2005-01-01
影响因子：
0.7
通讯作者：
Pourquié, O
中科院分区：
文献类型：
--
作者：
Maroto, M;Dale, JK;Pourquié, O
脊椎动物体轴的分割是在发育早期随着体体的顺序形成而开始的。体细胞发生是由一个分子振荡器暂时调节的，即分割时钟，它在体细胞前中胚层（PSM）细胞内起作用，驱动循环基因的周期性表达。我们研究了沿PSM循环基因表达进程的动力学。在这里，我们发现c-hairy1和c-hairy2 mRNA的表达以完全渐进的方式穿越PSM，并且这些基因和c- lng在每个周期中保持相似的表达前限。然而，关于这些基因的新振荡的开始和它们在尾侧PSM中的表达持续时间，我们看到了一些差异。我们还研究了PSM中振荡循环基因表达是否完全是细胞自主的。我们发现，虽然小的PSM外植体仍然能够维持它们的振荡时间表，但一旦它们被分离，PSM细胞就不再能够保持同步振荡。结果表明，细胞通讯或群落效应对这些细胞中循环基因的正常表达模式至关重要。
Segmentation of the vertebrate body axis is initiated early in development with the sequential formation of somites. Somitogenesis is temporally regulated by a molecular oscillator, the segmentation clock, which acts within presomitic mesoderm (PSM) cells to drive periodic expression of the cyclic genes. We have investigated the kinetics of the progression of cycling gene expression along the PSM. Here we show that c-hairy1 and c-hairy2 mRNA expression traverses the PSM in an entirely progressive manner and that both these genes and c-Lfng maintain a similar anterior limit of expression during each cycle. However, some differences are seen regarding both the onset of a new oscillation of these genes and the duration of their expression in the caudal PSM. We also investigated whether oscillating cyclic gene expression in the PSM is entirely cell autonomous. We find that while small PSM explants are still able to maintain their oscillation schedule, once they are dissociated, PSM cells are no longer able to maintain synchronous oscillations. The results imply that cell communication or a community effect is essential for the normal pattern of cyclic gene expression in these cells.