The trans-Golgi Network and the Golgi Stacks Behave Independently During Regeneration After Brefeldin A Treatment in Tobacco BY-2 Cells
The trans-Golgi Network and the Golgi Stacks Behave Independently During Regeneration After Brefeldin A Treatment in Tobacco BY-2 Cells
复制标题
DOI：
10.1093/pcp/pcx028
复制
发表时间：
2017-04-01
影响因子：
4.9
通讯作者：
Nakano, Akihiko
中科院分区：
文献类型：
--
作者：
Ito, Yoko;Toyooka, Kiminori;Nakano, Akihiko
跨高尔基体网络（TGN）在细胞内膜运输中发挥着重要作用。在植物细胞中，最近的活细胞成像研究揭示了 TGN 的动态行为独立于高尔基体。为了更好地了解这两个细胞器之间的关系，我们检查了它们对布雷菲德菌素 A (BFA) 试剂的动态响应以及去除 BFA 后的恢复情况。高尔基体标记物在一定浓度范围内对 BFA 的反应类似，而 TGN 的行为则依赖于 BFA 浓度。 TGN 在高浓度 BFA 下形成聚集体；然而，在较低的 BFA 浓度下，TGN 蛋白重新定位到分散在整个细胞质中的许多小囊泡结构。在弱 BFA 治疗恢复期间，TGN 早于高尔基体完成再生。两种细胞器的再生一度各自独立进行，最终通过它们的联合完成。我们的数据表明，烟草 BY-2 细胞中 TGN 和高尔基体的再生具有一定程度的自主性。
The trans-Golgi network (TGN) plays an essential role in intracellular membrane trafficking. In plant cells, recent live-cell imaging studies have revealed the dynamic behavior of the TGN independent from the Golgi apparatus. In order to better understand the relationships between the two organelles, we examined their dynamic responses to the reagent brefeldin A (BFA) and their recovery after BFA removal. Golgi markers responded to BFA similarly over a range of concentrations, whereas the behavior of the TGN was BFA concentration dependent. The TGN formed aggregates at high concentrations of BFA; however, TGN proteins relocalized to numerous small vesicular structures dispersed throughout the cytoplasm at lower BFA concentrations. During recovery from weak BFA treatment, the TGN started to regenerate earlier than the completion of the Golgi. The regeneration of the two organelles proceeded independently of each other for a while, and eventually was completed by their association. Our data suggest that there is some degree of autonomy for the regeneration of the TGN and the Golgi in tobacco BY-2 cells.