Murine Spleen Tissue Regeneration from Neonatal Spleen Capsule Requires Lymphotoxin Priming of Stromal Cells
Murine Spleen Tissue Regeneration from Neonatal Spleen Capsule Requires Lymphotoxin Priming of Stromal Cells
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DOI：
10.4049/jimmunol.1302115
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发表时间：
2014-08-01
影响因子：
4.4
通讯作者：
Watanabe, Takeshi
中科院分区：
文献类型：
--
作者：
Tan, Jonathan K. H.;Watanabe, Takeshi
脾是一种具有再生能力的组织，它允许自体移植人脾碎片来抵消脾切除术的影响。我们现在在小鼠模型中发现，仅移植新生儿脾被膜可导致完整脾组织的再生。这一发现表明，移植物来源的脾基质细胞，而不是淋巴细胞，是组织新生的重要组成部分，这一发现证实了移植和再生的Rag1KO脾囊。我们进一步证明，光氧素和淋巴组织诱导细胞参与脾新生的两个关键要素，大块组织再生和白色髓组织，确定一个光氧素依赖的途径新生儿脾再生，与以前定义的光氧素独立的胚胎脾器官形成对比。
Spleen is a tissue with regenerative capacity, which allows autotransplantation of human spleen fragments to counteract the effects of splenectomy. We now reveal in a murine model that transplant of neonatal spleen capsule alone leads to the regeneration of full spleen tissue. This finding indicates that graft-derived spleen stromal cells, but not lymphocytes, are essential components of tissue neogenesis, a finding verified by transplant and regeneration of Rag1KO spleen capsules. We further demonstrate that lymphotoxin and lymphoid tissue inducer cells participate in two key elements of spleen neogenesis, bulk tissue regeneration and white pulp organization, identifying a lymphotoxin-dependent pathway for neonatal spleen regeneration that contrasts with previously defined lymphotoxin-independent embryonic spleen organogenesis.