Mouse sperm lacking cell surface hyaluronidase PH-20 can pass through the layer of cumulus cells and fertilize the egg
Mouse sperm lacking cell surface hyaluronidase PH-20 can pass through the layer of cumulus cells and fertilize the egg
复制标题
DOI：
10.1074/jbc.m204596200
复制
发表时间：
2002-08-16
影响因子：
4.8
通讯作者：
Baba, T
中科院分区：
文献类型：
--
作者：
Baba, D;Kashiwabara, SI;Baba, T
糖基磷脂酰肌醇锚定的精子透明质酸酶PH-20在受精中的作用一直被认为是使顶体完整的精子能够穿过积云细胞层到达卵细胞透明带。在这项研究中，我们利用同源重组产生了携带PH-20基因零突变的小鼠。尽管缺乏PH-20的精子，突变的雄性小鼠仍然具有生育能力。体外受精实验表明，缺乏PH-20的小鼠精子从积云团中分散积云细胞的能力降低，导致仅在授精后的早期阶段延迟受精。此外，精子提取物的SDS-PAGE和随后的Western blot分析显示，除PH-20外，小鼠精子顶体中可能存在其他透明质酸酶。这些数据提供的证据表明，PH-20不是受精所必需的，至少在小鼠中，这表明其他透明质酸酶(s)可能在精子穿透积云细胞层和/或卵细胞透明带中发挥重要作用，可能与PH-20合作，尽管精子运动的重要性不容忽视。
The function of glycosylphosphatidylinositol-anchored sperm hyaluronidase PH-20 in fertilization has long been believed to enable acrosome-intact sperm to pass through the layer of cumulus cells and reach the egg zona pellucida. In this study, we have produced mice carrying a null mutation in the PH-20 gene using homologous recombination. Despite the absence of sperm PH-20, the mutant male mice were still fertile. In vitro fertilization assays showed that mouse sperm lacking PH-20 possess a reduced ability to disperse cumulus cells from the cumulus mass, resulting in delayed fertilization solely at the early stages after insemination. Moreover, SDS-PAGE of sperm extracts and subsequent Western blot analysis revealed the presence of other hyaluronidase(s), except PH-20, presumably within the acrosome of mouse sperm. These data provide evidence that PH-20 is not essential for fertilization, at least in the mouse, suggesting that the other hyaluronidase(s) may play an important role in sperm penetration through the cumulus cell layer and/or the egg zona pellucida, possibly in cooperation with PH-20, although the importance of sperm motility cannot be neglected.