Effects of Centrifugation on Cell Composition and Viability of Aspirated Adipose Tissue Processed for Transplantation
Effects of Centrifugation on Cell Composition and Viability of Aspirated Adipose Tissue Processed for Transplantation
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DOI：
10.1177/1090820x10369512
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发表时间：
2010-03-01
影响因子：
2.9
通讯作者：
Pitanguy, Ivo
中科院分区：
文献类型：
--
作者：
Conde-Green, Alexandra;Baptista, Leandra Santos;Pitanguy, Ivo
背景：离心是脂肪加工的首选方法之一。虽然在移植前分离脂肪组织成分已被推广了近三十年，但对于离心脂肪组织所获得的结果仍存在许多争议。 目的：作者证明离心对抽吸脂肪细胞成分的影响。方法：从 10 名接受抽脂的女性患者下腹部采集的脂肪分成两等份，然后通过倾析或离心处理并送至实验室。在苏木精和高碘酸希夫染色后对每个处理过的脂肪抽吸物进行组织学分析，以确定完整脂肪细胞的存在。然后进行培养并通过多色流式细胞仪进行分析，以鉴定脂肪来源的间充质干细胞。结果：许多外科医生使用的离心脂肪抽吸物的中层与倾析的样品相比显示出绝大多数改变的脂肪细胞和极少的间充质干细胞，这保持了脂肪细胞的完整性并显示出更多数量的间充质干细胞。在离心脂肪抽吸物底部观察到的第四层沉淀物显示出最大浓度的内皮细胞和间充质干细胞，它们在移植组织的血管生成和脂肪形成作用中起着至关重要的作用。结论：如果使用离心脂肪抽吸物，则应使用沉淀物（富含脂肪源性间充质干细胞）和中间层来增加脂肪移植物的存活率。
Background: Centrifugation is one of the preferred methods of fat processing. Although it has been promoted for nearly three decades to separate adipose tissue components before grafting, there remain many controversies regarding the results obtained with centrifuged adipose tissue.Objectives: The authors demonstrate the effects of centrifugation on the cellular components of aspirated fat.Methods: Fat harvested from the lower abdomen of 10 female patients undergoing liposuction was divided in two equal parts, then processed by decantation or centrifugation and sent to the laboratory. Each processed lipoaspirate was analyzed histologically after hematoxylin and periodic acid-Schiff staining for the presence of intact adipocytes. It was then cultured and analyzed by multicolor flow cytometry for identification of adipose-derived mesenchymal stem cells.Results: The middle layer of the centrifuged lipoaspirate, which is used by many surgeons, showed a great majority of altered adipocytes and very few mesenchymal stem cells in comparison with the decanted sample, which maintained the integrity of the adipocytes and showed a greater number of mesenchymal stem cells. The pellet observed as a fourth layer at the bottom of the centrifuged lipoaspirate showed the greatest concentration of endothelial cells and mesenchymal stem cells, which play a crucial role in the angiogenic and adipogenic effect of the grafted tissue.Conclusions: If centrifuged lipoaspirate is used, the pellet (rich in adipose-derived mesenchymal stem cells) and the middle layer should be employed to increase fat graft survival.