ESTABLISHMENT AND CHARACTERIZATION OF 7 DUNNING RAT PROSTATIC-CANCER CELL-LINES AND THEIR USE IN DEVELOPING METHODS FOR PREDICTING METASTATIC ABILITIES OF PROSTATIC CANCERS
ESTABLISHMENT AND CHARACTERIZATION OF 7 DUNNING RAT PROSTATIC-CANCER CELL-LINES AND THEIR USE IN DEVELOPING METHODS FOR PREDICTING METASTATIC ABILITIES OF PROSTATIC CANCERS
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DOI：
10.1002/pros.2990090306
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发表时间：
1986-01-01
期刊：
影响因子：
2.8
通讯作者：
SCHERES, J
中科院分区：
文献类型：
--
作者：
ISAACS, JT;ISAACS, WB;SCHERES, J
体外细胞系是从七个生物学上不同的体内 Dunning R3327 大鼠前列腺肿瘤亚系建立的。其中一些体外细胞系（即 G、AT-1、AT-2）在接种回同系哥本哈根雄性大鼠时保留了较低的转移能力，而其他细胞系（即 AT-3、MAT-LyLu、MAT-Lu）则保留了非常高的转移能力。使用一系列遗传参数（即每个细胞的DNA含量、众数染色体数）以及表型参数（即形态、5α-还原酶、雄激素受体、雌激素受体）来验证体外细胞系保留了用于其各自建立的亲本体内肿瘤亚系的主要特征。对高转移性体外细胞系与低转移性体外细胞系之间的一系列附加特征（即形态、生长速率、表面培养中的饱和密度、贴壁依赖性和独立的克隆形成潜力）进行比较，以确定是否可以识别可重复预测特定前列腺癌细胞系的转移能力的判别参数。虽然体外细胞系与其亲代体内肿瘤亚系的组合将成为开发预测前列腺癌转移能力的方法的宝贵工具，但尚未测量的单个参数能够完全成功地做出这一重要区分。
In vitro cell lines were established from seven biologically distinct in vivo Dunning R3327 rat prostatic tumor sublines. Some of these in vitro cell lines (i.e., G, AT-1, AT-2) retain a low metastatic ability when inoculated back into syngeneic Copenhagen male rats, while others (i.e., AT-3, MAT-LyLu, MAT-Lu) retain a very high metastatic ability. A series of genetic (i.e., DNA content per cell, modal chromosomal number), as well as phenotypic parameters, (i.e., morphology, 5.alpha.-reductase, androgen receptor, estrogen receptor) were used to validate that the in vitro cell lines retained the major characteristics of the parental in vivo tumor sublines used for their respective establishment. A series of additional characteristics (i.e., morphology, growth rate, saturation density in surface culture, anchorage-dependent and -independent clonogenic potential) were compared between the high vs. the low metastatic in vitro cell lines to determine if a discriminatory parameter could be identified which reproducibly predicted the metastatic abilities of the particular prostatic cancer cell line. While the combination of the in vitro cell lines and their parental in vivo tumor subline will be a valuable tool for developing methods for predicting metastatic ability of prostate cancers, no single parameter yet measured is entirely successful in making this important distinction.