Modified ELISPOT assay may predict T-cell hyporesponsiveness to non-inherited maternal antigens
Modified ELISPOT assay may predict T-cell hyporesponsiveness to non-inherited maternal antigens
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改良的 ELISPOT 检测可预测 T 细胞对非遗传性母体抗原的低反应性
DOI：
10.1111/j.1751-553x.2008.01121.x
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发表时间：
2010
影响因子：
3
通讯作者：
Saji H.
中科院分区：
文献类型：
--
作者：
Taniguchi K;Shimazaki C;Ochiai N;Maruya E;Akatsuka Y;Ashihara E;Maekawa T;Taniwaki M;Saji H.
临床报告表明，在人类白细胞抗原（HLA）不匹配的异基因干细胞移植（allo-SCT）中存在对非遗传性母体抗原（NIMA）的免疫耐受。我们使用IFN-γ酶联免疫斑点（ELISPOT）试验研究了3例HLA完全匹配的allo-SCT病例和1例健康志愿者家庭病例的T细胞反应性。在HLA完全匹配的异基因造血干细胞移植病例中，ELISPOT试验可检测供者T细胞对受者B细胞的低反应性。此外，ELISPOT测定显示，来自个体的T细胞对来自其母亲的B细胞的应答显著弱于来自无关HLA-半相合个体的T细胞。这些观察结果表明，我们基于IFN-γ ELISPOT测定的方法可以预测对NIMA的免疫耐受性的存在。
Clinical reports have suggested the existence of immunological tolerance to noninherited maternal antigens (NIMA) in human leukocyte antigen (HLA) mismatched allogeneic stem cell transplantation (allo‐SCT). We studied the T‐cell reactivity using IFN‐γ enzyme‐linked immunospot (ELISPOT) assay in three HLA fully matched allo‐SCT cases and one healthy volunteer family case. In HLA fully matched allo‐SCT cases, ELISPOT assay could detect the hyporesponsiveness of T cells from donors to the B cells from recipients. Moreover, ELISPOT assay showed that the T cells from an individual responded to B cell from his mother significantly weakly than those from an unrelated HLA‐haploidentical individual. These observations suggest that our IFN‐γ ELISPOT assay‐based method may predict the presence of immunological tolerance to NIMA.