Glucoamylase activity in industrial enzyme preparations using colorimetric enzymatic method: collaborative study.
Glucoamylase activity in industrial enzyme preparations using colorimetric enzymatic method: collaborative study.
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使用比色酶法测定工业酶制剂中的葡糖淀粉酶活性：合作研究。
DOI：
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发表时间：
1995
影响因子：
1.6
通讯作者：
R. Montgomery
中科院分区：
文献类型：
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作者：
M. Elder;R. Montgomery
15个实验室参与了一项合作研究，以验证工业酶制剂中葡糖淀粉酶测定的比色法。所有实验室均收到3家工业生产商提供的12份盲样。每份样品每天分析一次，持续3天。从统计分析中排除一个实验室的结果。根据AOAC指南计算了该方法的性能。对于所有15个实验室，重复性相对标准偏差（RSDr）值范围为0.89 - 2.37%，重现性相对标准偏差（RSDR）值范围为7.92 - 9.86。排除离群值后，RSDr值范围为0.74 - 2.24%，RSDR值范围为3.46 - 8.92。比色酶法测定工业酶制剂中的糖化酶活力已被AOAC国际首次采用。
Fifteen laboratories participated in a collaborative study to validate a colorimetric assay for the determination of glucoamylase in industrial enzyme preparations. All laboratories received 12 samples as blind duplicates provided by 3 industrial producers. Each sample was analyzed once a day for 3 days. Results from one laboratory were excluded from the statistical analysis. Performance of the method was calculated according to the AOAC guidelines. For all 15 laboratories the repeatability relative standard deviation (RSDr) values ranged from 0.89 to 2.37%, and the reproducibility relative standard deviation (RSDR) values ranged from 7.92 to 9.86. After excluding outliers the RSDr values ranged from 0.74 to 2.24%, and RSDR values ranged from 3.46 to 8.92. The colorimetric enzymatic method for determination of glucoamylase activity in industrial enzyme preparations has been adopted first action by AOAC INTERNATIONAL.