Immunogenic variants obtained by mutagenesis of mouse mastocytoma P815. V. H‐2 associativity of variant‐specific antigens
Immunogenic variants obtained by mutagenesis of mouse mastocytoma P815. V. H‐2 associativity of variant‐specific antigens
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通过小鼠肥大细胞瘤 P815 诱变获得的免疫原性变体。V. 变体特异性抗原的 H-2 关联性。
DOI：
10.1002/eji.1830121102
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发表时间：
1982
影响因子：
5.4
通讯作者：
Thierry Bonn
中科院分区：
文献类型：
--
作者：
J. Van Snick;J. Maryanski;A. van Pel;G. Parmiani;Thierry Bonn
通过肥大细胞瘤P815的体外诱变，有可能获得被同基因小鼠（tum-）排斥的肿瘤细胞变体。这些变体中的大多数携带新的个体抗原，并在混合白细胞肿瘤细胞培养物（MLTC）中刺激特异性溶细胞性T细胞（CTL）应答。通过测量针对H−2d复合物K或D末端产物的抗体对细胞介导的细胞溶解的抑制作用，检查了6种不同变体的该反应的H − 2相关性。裂解未被抑制（变体P91和P116）或被抗Kd（变体P21、P32和P198）或抗Dd抗体（变体P35）选择性抑制。通过H-2同种抗血清的吸收测定，所有这些tum−变体均表达Kd和Dd抗原。
By in vitro mutagenesis of mastocytoma P815, it is possible to obtain tumor cell variants that are rejected by syngeneic mice (tum−). Most of these variants carry new individual antigens and stimulate a specific cytolytic T cell (CTL) response in mixed leukocyte tumor cell culture (MLTC). The H‐2 associativity of this response was examined for six different variants by measuring the inhibition of cell‐mediated cytolysis by antibodies directed against products of the K or the D end of the H−2d complex. The lysis was either not inhibited (variants P91 and PI 16) or inhibited selectively by anti‐Kd (variants P21, P32 and P198) or anti‐Dd antibodies (variant P35). All these tum− variants expressed Kd and Dd antigens as measured by absorption of H‐2 alloantisera.