Significance of inoculation density and carbon source on the mycelial growth and Tuber polysaccharides production by submerged fermentation of Chinese truffle Tuber sinense
Significance of inoculation density and carbon source on the mycelial growth and Tuber polysaccharides production by submerged fermentation of Chinese truffle Tuber sinense
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DOI：
10.1016/j.procbio.2008.01.021
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发表时间：
2008-05-01
影响因子：
4.4
通讯作者：
Li, Hong-Mei
中科院分区：
文献类型：
--
作者：
Tang, Ya-Jie;Zhu, Ling-Li;Li, Hong-Mei
松露是市场上最有价值的美味蘑菇之一。以中华块菌为代表，首次建立了深层发酵生产块菌菌丝体和块菌多糖的工艺。研究了接种密度、碳源及碳源初始浓度等因素的影响。当接种密度在160-653 mg DW/L范围内时，在最低接种密度160 mg DW/L时获得最大生物量15.59 ± 0.59 g DW/L，而在最高接种密度653 mg DW/L时获得最大胞外多糖产量1.97 ± 0.08 g/L。在487 mg DW/L的水平下获得最大胞内多糖（IPS）产量1.40 +/- 0.10 g/L。所考察的碳源为葡萄糖、麦芽糖、蔗糖和乳糖，其中蔗糖最适合菌体生长和IPS的产生。乳糖有利于胞外多糖的产生，但不能使细胞生长良好。在乳糖培养基中，中华绒螯蟹生长过程中没有β-半乳糖苷酶活性，这是乳糖不利于细胞生长的原因。初始蔗糖浓度在20-125 g/L范围内对该过程有显著影响。在蔗糖125 g/L时，两种生物质（即，24.07 +/- 1.94 g/L）和EPS产量（即，2.85+/- 0.04 g/L）达到峰值。当蔗糖浓度为80 g/L时，IPS产量最大为2.92 +/- 0.20 g/L。本研究以中国块菌T. sinense是高效生产菌丝体和块茎多糖的潜在替代品。(C)2008爱思唯尔有限公司保留所有权利。
Truffles are among the most valuable gourmet mushrooms on the market. By taking Chinese truffle Tuber sinense as a typical example, a submerged fermentation process for the production of mycelia and Tuber polysaccharides was developed for the first time. Significances of inoculation density, carbon source and its initial concentration were studied in details. For inoculation density within the range of 160-653 mg dry weight (DW)/L, a maximal biomass of 15.59 +/- 0.59 g DW/L was obtained at its lowest level of 160 mg DW/L, while the maximal extracellular polysaccharides (EPSs) production of 1.97 +/- 0.08 g/L was attained at its highest level of 653 mg DW/L. The maximal intracellular polysaccharides (IPSs) production of 1.40 +/- 0.10 g/L was obtained at its level of 487 mg DW/L. The carbon sources examined were glucose, maltose, sucrose and lactose, and sucrose was suitable for the cell growth and IPS production. Lactose was beneficial for EPS production although the cell could not grow well. There was no P-galactosidase activity when T sinense grew in lactose, which was the reason why lactose was not favorable for the cell growth. Initial sucrose concentration within the range of 20-125 g/L significantly affected the process. At sucrose 125 g/L, both biomass (i.e., 24.07 +/- 1.94 g/L) and EPS production (i.e., 2.85 +/- 0.04 g/L) reached their peak values. The maximal IPS production of 2.92 +/- 0.20 g/L was obtained at sucrose 80 g/L. This work demonstrated the submerged fermentation of Chinese truffle T. sinense is a potential alternative for the efficient production of mycelia and Tuber polysaccharides. (C) 2008 Elsevier Ltd. All rights reserved.