Assembly of the Escherichia coli NADH:ubiquinone oxidoreductase (respiratory complex I)
Assembly of the Escherichia coli NADH:ubiquinone oxidoreductase (respiratory complex I)
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DOI：
10.1016/j.bbabio.2015.12.004
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发表时间：
2016-03-01
影响因子：
4.3
通讯作者：
Burschel, Sabrina
中科院分区：
文献类型：
--
作者：
Friedrich, Thorsten;Dekovic, Doris Kreuzer;Burschel, Sabrina
能量转换NADH：泛醌氧化还原酶，呼吸复合物I，将电子从NADH转移到泛醌与四个质子跨膜的易位偶联。大肠杆菌复合体I由13个不同的亚基组成，这些亚基由所谓的nuo基因编码。电子转移由9个辅因子、1个黄素单核苷酸和8个铁硫（Fe/S）簇催化。单个亚基和辅因子必须以协调的方式组装在一起，以保证活性全酶的生物发生。与线粒体相比，对细菌复合体的组装知之甚少。由于存在如此多的Fe/S-簇，组装的复合物I是密切相关的系统负责这些簇的生物起源。此外，据报道，在其他细菌中有效组装复合物需要一些其他蛋白质。建议的作用，已知的细菌组装因子进行了讨论，并从其他细菌物种的信息在此审查中使用绘制尽可能完整的模型，细菌复合物I组装。此外，还研究了该配合物在E.大肠杆菌中进行了简要描述。本文是由Conrad Mullineaux教授编辑的题为细菌生物能量系统的组织和动力学的特刊的一部分。(C)2015 Elsevier B. V.版权所有。
Energy-converting NADH:ubiquinone oxidoreductase, respiratory complex I, couples the electron transfer from NADH to ubiquinone with the translocation of four protons across the membrane. The Escherichia coli complex I is made up of 13 different subunits encoded by the so-called nuo-genes. The electron transfer is catalyzed by nine cofactors, a flavin mononucleotide and eight iron-sulfur (Fe/S)-clusters. The individual subunits and the cofactors have to be assembled together in a coordinated way to guarantee the biogenesis of the active holoenzyme. Only little is known about the assembly of the bacterial complex compared to the mitochondrial one. Due to the presence of so many Fe/S-clusters the assembly of complex I is intimately connected with the systems responsible for the biogenesis of these clusters. In addition, a few other proteins have been reported to be required for an effective assembly of the complex in other bacteria. The proposed role of known bacterial assembly factors is discussed and the information from other bacterial species is used in this review to draw an as complete as possible model of bacterial complex I assembly. In addition, the supramolecular organization of the complex in E. coli is briefly described. This article is part of a Special Issue entitled Organization and dynamics of bioenergetic systems in bacteria, edited by Prof. Conrad Mullineaux. (C) 2015 Elsevier B.V. All rights reserved.