Role of Mycobacterium tuberculosis copper-zinc superoxide dismutase
Role of Mycobacterium tuberculosis copper-zinc superoxide dismutase
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DOI：
10.1128/iai.69.1.529-533.2001
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发表时间：
2001-01-01
影响因子：
3.1
通讯作者：
Marchal, G
中科院分区：
文献类型：
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作者：
Dussurget, O;Stewart, G;Marchal, G
超氧化物歧化酶（SODs）在抵御氧化应激中起重要作用，并且已表明其对许多细菌种类的致病性有促进作用。为了确定分枝杆菌含铜和锌辅因子的超氧化物歧化酶（CuZnSOD）的功能，我们构建并鉴定了结核分枝杆菌和牛分枝杆菌卡介苗（BCG）的CuZnSOD缺失突变体。这两种菌株对超氧化物和过氧化氢比它们各自的亲本菌株更敏感。牛分枝杆菌卡介苗在未受刺激以及活化的小鼠骨髓来源巨噬细胞中的存活不受CuZnSOD缺失的影响。豚鼠组织中CuZnSOD缺陷型结核分枝杆菌的存活与其亲本菌株相当。这些结果表明，分枝杆菌的CuZnSOD对于巨噬细胞内的生长不是必需的，并且对结核分枝杆菌的致病性没有可检测到的贡献。
Superoxide dismutases (SODs) play an important role in protection against oxidative stress and have been shown to contribute to the pathogenicity of many bacterial species. To determine the function of the mycobacterial copper and zinc-cofactored SOD (CuZnSOD), we constructed and characterized Mycobacterium tuberculosis and Mycobacterium bovis BCG CuZnSOD null mutants. Both strains were more sensitive to superoxides and hydrogen peroxide than were their respective parental strains. The survival of M. bovis BCG in unstimulated as well as activated mouse bone marrow-derived macrophages was not affected by the loss of CuZnSOD. The survival of CuZnSOD deficient-M. tuberculosis in guinea pig tissues was comparable to that of its parental strain. These results indicate that the mycobacterial CuZnSOD is not essential for intracellular growth within macrophages and does not detectably contribute to the pathogenicity of M. tuberculosis.