Population Structure of Multidrug-Resistant Klebsiella oxytoca within Hospitals across the United Kingdom and Ireland Identifies Sharing of Virulence and Resistance Genes with K-pneumoniae
Population Structure of Multidrug-Resistant Klebsiella oxytoca within Hospitals across the United Kingdom and Ireland Identifies Sharing of Virulence and Resistance Genes with K-pneumoniae
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DOI：
10.1093/gbe/evx019
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发表时间：
2017-03-01
影响因子：
3.3
通讯作者：
Parkhill, Julian
中科院分区：
文献类型：
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作者：
Moradigaravand, Danesh;Martin, Veronique;Parkhill, Julian
产酸克雷伯菌是肠杆菌科的成员，是一种环境来源的革兰氏阴性致病菌，可在医疗机构中引起感染。住院患者中越来越多地报道了耐多药产酸克雷伯菌感染的爆发。尽管这种病原体的重要性日益增加，但人们对抗菌素耐药克雷伯氏菌的种群结构和流行病学的了解仍然有限。我们研究了从英国和爱尔兰的血流感染中回收的 41 种多重耐药产酸克雷伯菌分离株的群体结构和抗菌素耐药性的基因组基础。我们的结果表明，K. oxytoca 具有高度多样化的种群，由几个不同的分支组成，并且我们在数据集中发现了一个最近的克隆扩展。尽管产酸克雷伯氏菌基因组明显不同于主要由肺炎克雷伯氏菌组成的全球肺炎克雷伯氏菌复合体的基因组，但我们发现了通过重组共享核心基因的证据，以及物种之间辅助抗菌素耐药性和毒力因子基因的交换。我们的研究结果还表明，不同的产酸克雷伯氏菌分支已独立获得抗菌素耐药性和毒力因子基因。这凸显了产酸克雷伯氏菌遗传灵活性的临床和治疗重要性以及其作为机会病原体的相关性。
Klebsiella oxytoca, a member of the Enterobacteriaceae, is a gram-negative pathogenic bacterium of environmental origin, which can cause infection in healthcare settings. Outbreaks of multidrug-resistant K. oxytoca infection have been increasingly reported in hospitalized patients. Despite the growing importance of this pathogen, there is limited knowledge about the population structure and epidemiology of antimicrobial resistant K. oxytoca. We investigated the population structure and genomic basis of antimicrobial resistance of 41 multidrug resistant K. oxytoca isolates recovered from bloodstream infections across the UK and Ireland. Our results show that K. oxytoca has a highly diverse population, which is composed of several distinct clades, and we identified one recent expansion of a clone within our dataset. Although the K. oxytoca genomes are clearly distinct from the genomes of a global collection of Klebsiella pneumoniae complex, pre-dominantly composed of K. pneumoniae, we found evidence for sharing of core genes through recombination, as well as the exchange of accessory antimicrobial resistance and virulence factor genes between the species. Our findings also suggest that the different K. oxytoca clades have acquired antimicrobial resistance and virulence factor genes independently. This highlights the clinical and therapeutic importance of genetic flexibility in K. oxytoca and the relevance of this in its role as an opportunistic pathogen.