Progesterone secretion by corpora lutea of the isolated perfused rabbit ovary during pseudopregnancy.
Progesterone secretion by corpora lutea of the isolated perfused rabbit ovary during pseudopregnancy.
复制标题
假妊娠期间离体灌注兔卵巢黄体分泌的黄体酮。
DOI：
10.1095/biolreprod38.5.1137
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发表时间：
1988
影响因子：
3.6
通讯作者：
Wallach,EE
中科院分区：
文献类型：
--
作者：
Dharmarajan,AM;Yoshimura,Y;Sueoka,K;Atlas,SJ;Dubin,NH;Ewing,LL;Zirkin,BR;Wallach,EE
采用离体卵巢灌注法，观察了人工流产过程中家兔黄体（CL）的功能。用含或不含3%牛血清白蛋白（BSA）的组织培养液199对卵巢进行体外灌注。从动脉和静脉插管中获取样本，以确定孕酮分泌速率。两种灌注方法进行了比较：一个封闭的系统中，灌注介质不断通过组织再循环，和一个开放的系统中，卵巢静脉插管被留在灌注室外。发现添加3%BSA可防止水肿和间质空间的扭曲，并导致孕酮分泌增加。与封闭的灌注系统，孕激素分泌率测量超过6小时灌注显着高于第11天比第1天的假孕，并在第18天显着下降。无论灌注系统是开放的还是封闭的，分泌速率都没有差异，两种系统获得的结果非常接近体内孕酮分泌速率。CL组织孕酮含量的测量之前和之后，在体外灌注表明，孕酮分泌的变化，在假孕激素的合成和分泌的差异，而不是从泄漏的孕酮已经存在于CL灌注前导致。总之，这些结果表明，本文所述的改良的体外灌注兔卵巢制备物是检查携带CL的卵巢的孕酮分泌的适当模型。
An ovarian in vitro perfusion method was adapted to examine rabbit corpus luteum (CL) function during pseudopregnancy. Ovaries were perfused in vitro with tissue culture Medium 199 with or without 3% bovine serum albumin (BSA). Samples were obtained from both arterial and venous cannulae, allowing rates of progesterone secretion to be determined. Two perfusion methods were compared: a closed system in which perfusion medium continuously recirculated through the tissue, and an open system in which the ovarian vein cannula was left outside the perfusion chamber. The addition of 3% BSA was found to prevent edema and distortion of the interstitial space, and to result in increased progesterone secretion. With the closed perfusion system, the progesterone secretion rate measured over the 6-h perfusion was significantly higher on Day 11 than on Day 1 of pseudopregnancy and had declined significantly on Day 18. There was no difference in secretion rates whether the perfusion system was open or closed, and the results obtained with both systems closely approximated in vivo progesterone secretion rates. Measurements of CL tissue progesterone content before and after in vitro perfusion indicated that the changes in progesterone secretion seen during pseudopregnancy resulted from differences in the synthesis and secretion of progesterone and not from leakage of progesterone already present in the CL prior to perfusion. Taken together, these results indicate that the modified in vitro perfused rabbit ovary preparation described herein is an appropriate model to examine progesterone secretion by ovaries bearing CL.