Effects of Er: YAG laser irradiation on wettability, surface roughness, and biocompatibility of SLA titanium surfaces: an in vitro study
Effects of Er: YAG laser irradiation on wettability, surface roughness, and biocompatibility of SLA titanium surfaces: an in vitro study
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DOI：
10.1007/s10103-013-1361-y
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发表时间：
2015-02-01
影响因子：
2.1
通讯作者：
Safar-Hajhosseini, Ali
中科院分区：
文献类型：
--
作者：
Ayobian-Markazi, Nader;Karimi, Mohammadreza;Safar-Hajhosseini, Ali
Er：Y3+石榴石(Er：YAG)激光是一种有效的种植体表面去污方法。关于Er：YAG激光对钛的生物和表面性质的影响的数据相互矛盾。表面性质对细胞行为有很大的影响，包括钛表面的成分、粗糙度、润湿性和表面形态。本研究的目的是研究Er：YAG激光对喷砂和酸蚀(SLA)钛表面生物相容性、表面粗糙度和润湿性的影响。用脉冲能量为100mJ、脉冲频率为10 Hz的Er：YAG激光对21个SLA钛圆盘进行激光照射，时间为1min。对细胞存活率、表面粗糙度和润湿性变化进行了评估。13个未照射的SLA圆盘作为对照组。将人成骨样SAOS-2细胞接种于培养基盘上。用四甲基偶氮唑蓝比色法检测细胞存活率。使用轮廓仪和张力计分别测量了试验组和对照组的表面粗糙度和润湿性。实验组的细胞存活率显著高于对照组(p=0.032)。与对照组相比，试验组表面粗糙度显著降低(p=0.008)。试验组的表面润湿性显著高于对照组(p=0.004)。在本研究的范围内，应用具有上述特性的Er：YAG激光似乎没有对SLA钛表面的生物相容性产生不利影响。激光的应用降低了SLA钛表面的表面粗糙度，增加了表面的润湿性。
The erbium: yttrium-aluminum-garnet (Er: YAG) laser has been introduced as an effective method in the decontamination of implant surfaces. Data concerning the effects of the Er: YAG laser on the biological and surface properties of titanium are conflicting. Cellular behavior is greatly affected by surface properties, including composition, roughness, wettability, and morphology of the titanium surface. The aim of this study was to investigate the influence of the Er: YAG laser on the biocompatibility, surface roughness, and wettability of sandblasted and acid-etched (SLA) titanium surfaces. Twenty-one SLA titanium disks were irradiated by the Er: YAG laser at a pulse energy of 100 mJ, with a pulse frequency of 10 Hz under water irrigation for 1 min. Cell viability, surface roughness, and wettability alterations were evaluated. Thirteen nonirradiated SLA disks were used as the control groups. Human osteoblast-like SaOs-2 cells were seeded onto the disks in culture media. Cell viability was evaluated using the methylthiazol tetrazolium assay. The surface roughness and wettability of the test and control groups were measured using profilometer and tensiometer devices, respectively. A significantly higher cell viability rate was observed in the test group (p = 0.032). The surface roughness was significantly reduced in the test group compared with the control group (p = 0.008). The surface wettability was significantly higher in the test group (p = 0.004). Within the limits of this study, the application of the Er: YAG laser with the previously described properties did not appear to have adverse effects on the biocompatibility of the SLA titanium surfaces. Application of this laser decreased the surface roughness and increased the wettability of the SLA titanium surfaces.