Expression and characteristics of rice xylanase inhibitor OsXIP, a member of a new class of antifungal proteins
Expression and characteristics of rice xylanase inhibitor OsXIP, a member of a new class of antifungal proteins
复制标题
DOI：
10.1007/s10535-018-0787-2
复制
发表时间：
2018-09
影响因子：
1.5
通讯作者：
Renjie Sun;Yanxia Xu;Chunxiao Hou;Yi-hua Zhan;M.-Q. Liu;X. Weng
中科院分区：
文献类型：
--
作者：
Renjie Sun;Yanxia Xu;Chunxiao Hou;Yi-hua Zhan;M.-Q. Liu;X. Weng
据推测，木聚糖酶抑制剂在植物防御微生物病原体中起重要作用。目前，关于木聚糖酶抑制剂OsXIP在水稻中的表达及其相关基因的研究还很少。我们从水稻（Oryza sativa L.）CV. Nipponbare）基因组DNA。为了确定OsXIP的功能，我们获得了过量表达OsXIP的转基因水稻植株。与野生型相比，转基因植株OsXIP表达量显著增加，对稻瘟病菌的防御反应增强。OsXIP表达的增加伴随着病程相关基因的上调。为了明确OsXIP的表达模式，构建了ProOsXIP：：GUS载体，获得了转基因植株。GUS染色结果表明，OsXIP在水稻植株中具有器官特异性表达，主要在根和叶脉中表达，而在叶片中表达较弱。OsXIP在生物胁迫和非生物胁迫下的表达分析表明，生物胁迫和茉莉酸甲酯处理显著诱导了OsXIP的表达。OsXIP是一类新的抗真菌蛋白，可能作为一种屏障，阻止真菌病原体分泌的木聚糖酶降解细胞壁。OsXIP基因是一个逆境应答基因，可以通过茉莉酸介导的信号途径参与植物的防御。
It has been hypothesized that xylanase inhibitors play important roles in plant defense against microbial pathogens. Currently, there is little information available about xylanase inhibitor OsXIP in rice and its gene expression. We cloned a xylanase inhibitor geneOsXIPfrom rice (Oryza sativaL. cv. Nipponbare) genomic DNA. To determine the function of OsXIP, we generatedOsXIP-overexpressing transgenic rice plants. The transgenic plants had significantly higherOsXIPexpression and showed enhanced defense response toMagnaporthe oryzaecompared to the wild-type plants. The results also showed that the increasedOsXIPexpression was accompanied by the up-regulation of pathogenesisrelated genes. To clarify theOsXIPexpression pattern, aProOsXIP::GUSvector was constructed and transgenic plants were obtained. GUS staining results revealed thatOsXIPshowed organ-specific expressions in rice plants.OsXIPwas primarily expressed in the roots and in the veins, but it was weakly expressed in the leaves. Analyses of theOsXIPexpression in response to biotic and abiotic stresses indicated that it was drastically induced by biotic stresses and methyl jasmonate treatment. OsXIP, a member of a new class of antifungal proteins, may function as a barrier that prevents the cell wall degradation by xylanases excreted by fungal pathogens. TheOsXIPwas found to be a stressresponsive gene and it could take part in plant defenseviaa JA-mediated signaling pathway.