Effects of dietary levels of 20:5n-3 and 22:6n-3 on tissue lipid composition in juvenile Atlantic salmon, Salmo salar, with emphasis on brain and eye
Effects of dietary levels of 20:5n-3 and 22:6n-3 on tissue lipid composition in juvenile Atlantic salmon, Salmo salar, with emphasis on brain and eye
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DOI：
10.1046/j.1365-2095.1997.00085.x
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发表时间：
1997-09-01
影响因子：
3.5
通讯作者：
Lie, O.
中科院分区：
文献类型：
--
作者：
Brodtkorb, T.;Rosenlund, G.;Lie, O.
分别饲喂4组大西洋鲑鱼幼鱼，每组3个重复，分别提高二十二碳六烯酸（22:6n-3； DHA）和二十碳五烯酸（20:5n-3； EPA）水平。在试验开始时和试验期间大约每3周测定一次鱼脑和眼的脂肪酸组成，在最终取样时测定鱼肝和鱼片的脂肪酸组成。实验结束时测定脑和眼的脂类组成及其脂肪酸组成。饲料中DHA含量的增加对大西洋鲑鱼幼鱼脑中脂肪酸组成、脂类组成和脂类DHA水平均无影响。饲料中EPA含量的增加既体现在总脂肪酸组成上，也体现在中性脂、磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）和磷脂酰肌醇（PI）中的EPA含量上。在鲑鱼幼鱼眼的脂质组成中发现了膳食DHA含量增加的轻微影响。而眼内EPA和18:2n-6水平分别清晰地反映了日粮中这两种脂肪酸水平的增加和减少。饲料中EPA水平对鲑鱼幼鱼眼中性脂、PC、PE、PI和PS（磷脂酰丝氨酸）中的EPA水平也有影响。结果表明，这些膳食水平的DHA对脑脂质的组成没有影响，对眼脂质的组成只有轻微的影响。此外，饲料中EPA水平显著影响了脑和眼的脂质组成。鱼片脂肪酸组成反映了饲料脂肪酸组成，但鱼片中的DHA/EPA比例与饲料中的相反。肝脏脂肪酸组成也受到饲料中EPA和DHA水平增加的影响。
Four dietary groups of juvenile Atlantic salmon, Salmo salar L., each with three replicates, were fed diets with increasing levels of docosahexaenoic acid (22:6n-3; DHA) and eicosapentaenoic acid (20:5n-3; EPA). Fatty acid composition of brain and eye was determined at the start and approximately every 3 weeks during the experimental period, and fatty acid composition of liver and fillet was determined in fish from the final sampling. Lipid class composition of brain and eye, and fatty acid composition of these lipid classes was determined at the end of the experiment. There was no effect of increasing dietary DHA content on fatty acid composition, lipid class composition or DHA levels in the lipid classes in the juvenile Atlantic salmon brain. The increasing dietary EPA content, however, was reflected in both the total fatty acid composition and in the EPA content in neutral lipids, phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI). A minor effect of the increasing dietary DHA content was found in the lipid composition of the juvenile salmon eye. Both EPA and 18:2n-6 levels in eye, however, clearly reflected the increasing and decreasing, respectively, dietary levels of these two fatty acids. The dietary EPA levels also affected the EPA levels in neutral lipids, PC, PE, PI and PS (phosphatidylserine) in the juvenile salmon eye. The results demonstrate that these dietary levels of DHA had no effect on brain lipid composition and only a minor effect on eye lipid composition. Furthermore, the dietary EPA levels significantly affected the lipid composition of both brain and eye. The fillet fatty acid composition reflected the dietary fatty acid composition, except for the DHA/EPA ratio, which was reversed in fillet compared with that in the diets. The liver fatty acid composition was also affected by the increasing dietary EPA and DHA levels.