Mapping evolution with ribosome structure: intralineage constancy and interlineage variation.
Mapping evolution with ribosome structure: intralineage constancy and interlineage variation.
复制标题
用核糖体结构绘制进化图：谱系内恒定性和谱系间变异。
DOI：
10.1073/pnas.79.19.5948
复制
发表时间：
1982
影响因子：
11.1
通讯作者：
Matheson,AT
中科院分区：
文献类型：
--
作者：
Lake,JA;Henderson,E;Clark,MW;Matheson,AT
核糖体小亚基有三种结构模式，分别对应于真细菌、考古细菌和真核生物的谱系。在这些谱系中，核糖体结构高度保守。来自所有三个谱系的小亚基有一个共同的整体结构，除了下列不同之处：(1)来自考古菌和真核生物细胞质成分的小亚基都在亚单位的头部包含一个特征，即真核细菌票据，这在真核细菌中没有；(2)真核小亚单位在亚单位的底部包含额外的密度区域，这在考古细菌和真核细菌中是不存在的。我们将核糖体三维结构的系内保守性解释为将考古细菌、真细菌和真核生物视为原始谱系的系统发育基础。虽然我们的数据与Woese和Fox的数据是分开和独立的，但它们进一步支持了他们的建议[Woese，C.R.&Fox，G.E.(1977)Proc.娜塔莉。阿卡德。SCI。美国74,5088-5090]。这些数据还为生物体分类和识别新的谱系提供了一种简单、快速和准确的方法。最后，核糖体结构的谱系间变异被用来建立一个严格的框架来考虑这三个品系的进化。
Ribosomal small subunits are organized in three general structural patterns that correspond to the eubacterial, archaebacterial, and eukaryotic lineages. Within each of these lineages, ribosomal structure is highly conserved. Small subunits from all three lineages share a common overall structure except for the following differences: (i) small subunits from archaebacteria and from the cytoplasmic component of eukaryotes both contain a feature on the head of the subunit, the archaebacterial bill, that is absent in eubacteria, and (ii) eukaryotic small subunits contain additional regions of density at the base of the subunit, the eukaryotic lobes, that are absent in archaebacteria and in eubacteria. We interpret the intralineage conservation of ribosomal three-dimensional structure as forming a phylogenetic basis for regarding archaebacteria, eubacteria, and eukaryotes as primitive lines. Although our data are separate and independent from those of Woese and Fox, they lend further support to their proposal [Woese, C. R. & Fox, G. E. (1977) Proc. Natl. Acad. Sci. USA 74, 5088-5090]. These data also provide a simple, rapid, and accurate method for classifying organisms and for identifying new lineages. Finally, interlineage variation of ribosomal structure is used to establish a rigorous framework for considering the evolution of these three lines.