Preparation of rodent hippocampal slice cultures.
Preparation of rodent hippocampal slice cultures.
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DOI：
10.1101/pdb.prot4848
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发表时间：
2007-10-01
期刊：
影响因子：
--
通讯作者：
Dailey, Michael E
中科院分区：
文献类型：
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作者：
Fuller, Leah;Dailey, Michael E
引言啮齿类动物海马脑片培养物（OHSCs）为研究神经元和神经胶质细胞在其自然三维环境中的动态结构和生理特征提供了一种出色的中枢神经系统组织制备方法。从新生啮齿类动物获得组织切片是一件简单的事情。这些切片培养良好，长达数周，易于操作，允许各种体外实验模型。OHSC为涉及活细胞成像的研究提供了良好的光学和生理可及性，具有高空间和时间分辨率。该方案用于收获固定后用于免疫组织化学标记的组织，以及用于通过各种荧光染料或通过生物射弹或病毒转染标记的活组织中的共聚焦延时成像。
INTRODUCTIONRodent organotypic hippocampal slice cultures (OHSCs) provide an outstanding preparation of central nervous system tissue for exploring the dynamic structural and physiological features of neuronal and glial cells within their native three-dimensional environments. It is a straightforward matter to obtain tissue slices from neonatal rodents. These slices culture well for periods up to several weeks and are easy to manipulate, allowing for a variety of in vitro experimental models. OHSCs provide good optical and physiological accessibility for studies involving live cell imaging, with high spatial and temporal resolution. This protocol is used to harvest tissues for both immunohistochemical labeling after fixation, and for confocal time-lapse imaging in live tissues labeled by a variety of fluorescent dyes or by biolistic or viral transfection.