EXPRESSION OF HUMAN BONE MORPHOGENETIC PROTEINS-2 OR PROTEINS-4 IN MURINE MESENCHYMAL PROGENITOR C3H10T1/2 CELLS INDUCES DIFFERENTIATION INTO DISTINCT MESENCHYMAL CELL LINEAGES
EXPRESSION OF HUMAN BONE MORPHOGENETIC PROTEINS-2 OR PROTEINS-4 IN MURINE MESENCHYMAL PROGENITOR C3H10T1/2 CELLS INDUCES DIFFERENTIATION INTO DISTINCT MESENCHYMAL CELL LINEAGES
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DOI：
10.1089/dna.1993.12.871
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发表时间：
1993-12-01
影响因子：
3.1
通讯作者：
GROSS, G
中科院分区：
文献类型：
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作者：
AHRENS, M;ANKENBAUER, T;GROSS, G
将真核表达载体中编码人骨形态发生蛋白BMP-2和BMP-4的cDNA永久转移到小鼠间充质祖细胞系C3 H10 T1/2中。最初，已知这些细胞在加入氮杂胞苷后分化成肌管、脂肪细胞和软骨细胞。编码人BMP-2和BMP-4的基因的永久转染诱导分化为成骨谱系。C3 H10 T1/2细胞的成骨分化潜能通过骨生成的典型或特异性标记基因的组织化学和遗传分析来证实，所述标记基因包括甲状旁腺激素受体、碱性磷酸酶、骨桥蛋白、骨连接蛋白和骨钙蛋白。除了成骨细胞形成之外，还观察到发育成脂肪细胞和软骨细胞，提示BMP-2和BMP-4诱导分化成三种间充质谱系。
The cDNAs encoding the human bone morphogenetic proteins BMP-2 and BMP-4 in an eukaryotic expression vector were permanently transferred into the murine mesenchymal progenitor cell line C3H10T1/2. Originally, these cells are known to differentiate into myotubes, adipocytes, and chondrocytes upon the addition of azacytidine. Permanent transfection of genes encoding human BMP-2 and BMP-4 induces differentiation into the osteogenic lineage. The osteogenic differentiation potential of C3H10T1/2 cells is substantiated by histochemical and genetic analyses of marker genes typical or specific for osteogenesis, including the parathyroid hormone receptor, alkaline phosphatase, osteopontin, osteonectin, and osteocalcin, In addition to osteoblast formation, development into adipocytes and chondrocytes is also observed, suggesting that BMP-2 and BMP-4 induce differentiation into three mesenchymal lineages.